<abstract xmlns="http://www.w3.org/1999/xhtml"><p> During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofloxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, while all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health.</p></abstract>

During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofloxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, while all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health.

Antibiotic Resistance and Molecular Studies on Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated in Human Cases and Broiler Carcasses

Institute of Meat Hygiene and Technology, Kaćanskog 13, Belgrade, Serbia

Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, Belgrade, Serbia

Veterinary Specialized Institute, “Кraljevo”, Žička 34, Kraljevo, Serbia

Acta Veterinaria

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150...

Antibiotic Resistance and Molecular Studies on Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated in Human Cases and Broiler Carcasses

Published Online: Jun 17, 2014

Page range: 257 - 268

Received: Jan 20, 2014

Accepted: Mar 13, 2014

DOI: https://doi.org/10.2478/acve-2014-0024

Keywords
Salmonellosis, prevalence of Salmonella on broiler carcasses, Salmonella Infantis, antibiotic resistance, PFGE

© by Mladen Rašeta

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Antibiotic Resistance and Molecular Studies on Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated in Human Cases and Broiler Carcasses

Published Online: Jun 17, 2014

Page range: 257 - 268

Received: Jan 20, 2014

Accepted: Mar 13, 2014

DOI: https://doi.org/10.2478/acve-2014-0024

KeywordsSalmonellosis, prevalence of Salmonella on broiler carcasses, Salmonella Infantis, antibiotic resistance, PFGE

© by Mladen Rašeta

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords
Salmonellosis, prevalence of Salmonella on broiler carcasses, Salmonella Infantis, antibiotic resistance, PFGE