<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.</p></abstract>

In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;

Veterinary Specialist Institute “Kraljevo”, Zicka 34, Kraljevo, Serbia;

Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, Belgrade, Serbia;

Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, Serbia.

Acta Veterinaria

In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus...

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Published Online: Mar 25, 2014

Page range: 81 - 92

DOI: https://doi.org/10.2478/acve-2014-0009

Keywords
DWV, ABPV, Varroa destructor, Serbian apiaries

This content is open access.

A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

Published Online: Mar 25, 2014

Page range: 81 - 92

DOI: https://doi.org/10.2478/acve-2014-0009

KeywordsDWV, ABPV, Varroa destructor, Serbian apiaries

This content is open access.

Keywords
DWV, ABPV, Varroa destructor, Serbian apiaries