<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study, <italic>b</italic>-cyclodextrin was used as a chiral selector for the recognition of panthenol enantiomers. Inclusion complexes formed by <italic>b</italic>-cyclodextrin and the analyte showed small differences in NIR and UV/VIS spectra compared by chemometric assessment. Based on the figure of merit and model characteristics, PLS calibration model in the selected range of NIR spectra is preferred. UV/VIS spectrometry has the disadvantage of complicated sample preparation compared to NIR spectrometry. Results reached by both proposed methods were in good agreement. Statistical investigation of the results (by Student <italic>t</italic>-test and Fisher <italic>F</italic>-test) confirmed that the proposed methods are comparable and applicable to chiral analysis of panthenol pharmaceutical preparations.</p></abstract>

Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study, b-cyclodextrin was used as a chiral selector for the recognition of panthenol enantiomers. Inclusion complexes formed by b-cyclodextrin and the analyte showed small differences in NIR and UV/VIS spectra compared by chemometric assessment. Based on the figure of merit and model characteristics, PLS calibration model in the selected range of NIR spectra is preferred. UV/VIS spectrometry has the disadvantage of complicated sample preparation compared to NIR spectrometry. Results reached by both proposed methods were in good agreement. Statistical investigation of the results (by Student t-test and Fisher F-test) confirmed that the proposed methods are comparable and applicable to chiral analysis of panthenol pharmaceutical preparations.

Spectro-chemometric determination of panthenol enantiomeric excess in pharmaceutical products

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active...

Spectro-chemometric determination of panthenol enantiomeric excess in pharmaceutical products

Published Online: Jan 29, 2021

Page range: 23 - 29

DOI: https://doi.org/10.2478/acs-2020-0020

Keywords
chiral analysis, panthenol, pharmaceutical preparations, molecular spectrometry, multivariate calibration

© 2020 Roman Poláček et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Spectro-chemometric determination of panthenol enantiomeric excess in pharmaceutical products

Published Online: Jan 29, 2021

Page range: 23 - 29

DOI: https://doi.org/10.2478/acs-2020-0020

Keywordschiral analysis, panthenol, pharmaceutical preparations, molecular spectrometry, multivariate calibration

© 2020 Roman Poláček et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
chiral analysis, panthenol, pharmaceutical preparations, molecular spectrometry, multivariate calibration