<abstract xmlns="http://www.w3.org/1999/xhtml">Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5µM, ANXA1 induced significant apoptosis in K562 (<italic>p</italic> ≤ 0.001) and U937 (<italic>p</italic> ≤ 0.05) cells, with <italic>EC</italic>50 values of 3.6 and 3.8 µM, respectively. In Jurkat cells, induction was not significant (<italic>EC</italic>50, 17.0 µM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with <italic>p</italic> ≤ 0.001 for both, and (<italic>p</italic> ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.</abstract>

Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell cycle arrest in K562, Jurkat and U937 cells and peripheral blood mononu-clear cells (PBMC). Cells were treated with ANXA1 and cyclophosphamide prior to flow cytometry analysis for apoptosis and cell cycle arrest induction. At 2.5µM, ANXA1 induced significant apoptosis in K562 (p ≤ 0.001) and U937 (p ≤ 0.05) cells, with EC50 values of 3.6 and 3.8 µM, respectively. In Jurkat cells, induction was not significant (EC50, 17.0 µM). No significant apoptosis induction was observed in PBMC. ANXA1 caused cycle arrest in the G0/G1 phase in K562 and U937 cells with p ≤ 0.001 for both, and (p ≤ 0.01) for Jurkat cells. ANXA1 induced apoptosis and cycle arrest in the G0/G1 phase in K562 and U937 cells, causing only cell cycle arrest in Jurkat cells.

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Drug and Herbal Research Centre Faculty of Pharmacy

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Recent studies suggest that annexin A1 (ANXA1) promotes apoptosis in cancerous cells. This study aims to investigate the effects of ANXA1 on apoptosis and cell...

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Published Online: Dec 07, 2018

Page range: 75 - 86

Accepted: Aug 20, 2018

DOI: https://doi.org/10.2478/acph-2019-0005

Keywords
annexin A1, apoptosis, cell cycle, flow cytometry, human leukemic cell lines

© 2019 Affidah Sabran et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines

Published Online: Dec 07, 2018

Page range: 75 - 86

Accepted: Aug 20, 2018

DOI: https://doi.org/10.2478/acph-2019-0005

Keywordsannexin A1, apoptosis, cell cycle, flow cytometry, human leukemic cell lines

© 2019 Affidah Sabran et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
annexin A1, apoptosis, cell cycle, flow cytometry, human leukemic cell lines