<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Correlation analysis based on chromatograms and pharmacological activities is essential for understanding the effective components in complex herbal medicines. In this report, HPLC and measurement of antioxidant properties were used to describe the active ingredients of Salvia miltiorrhiza injection (SMI). HPLC results showed that tanshinol, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, protocatechuic acid and their metabolites in rat serum may contribute to the efficacy of SMI. Assessment of antioxidant properties indicated that differences in the composition of serum powder of SMI caused differences in vascular endothelial cell protection. When bivariate correlation was carried out it was found that salvianolic acid B, tanshinol and protocatechuic aldehyde were active components of SMI because they were correlated to antioxidant properties.</p></abstract>

Correlation analysis based on chromatograms and pharmacological activities is essential for understanding the effective components in complex herbal medicines. In this report, HPLC and measurement of antioxidant properties were used to describe the active ingredients of Salvia miltiorrhiza injection (SMI). HPLC results showed that tanshinol, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, protocatechuic acid and their metabolites in rat serum may contribute to the efficacy of SMI. Assessment of antioxidant properties indicated that differences in the composition of serum powder of SMI caused differences in vascular endothelial cell protection. When bivariate correlation was carried out it was found that salvianolic acid B, tanshinol and protocatechuic aldehyde were active components of SMI because they were correlated to antioxidant properties.

Analysis of Active Components in Salvia Miltiorrhiza Injection Based on Vascular Endothelial Cell Protection

The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou Zhejiang Province – 310 006, China

The First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou Zhejiang Province – 310 003, China

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Correlation analysis based on chromatograms and pharmacological activities is essential for understanding the effective components in complex herbal medicines....

Analysis of Active Components in Salvia Miltiorrhiza Injection Based on Vascular Endothelial Cell Protection

Published Online: Oct 08, 2014

Page range: 325 - 334

Accepted: May 14, 2014

DOI: https://doi.org/10.2478/acph-2014-0027

Keywords
Salvia miltiorrhiza injection, HPLC, antioxidant activity, correlation analysis, salvianolic acid

© by Minxia Zheng

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Analysis of Active Components in Salvia Miltiorrhiza Injection Based on Vascular Endothelial Cell Protection

Published Online: Oct 08, 2014

Page range: 325 - 334

Accepted: May 14, 2014

DOI: https://doi.org/10.2478/acph-2014-0027

KeywordsSalvia miltiorrhiza injection, HPLC, antioxidant activity, correlation analysis, salvianolic acid

© by Minxia Zheng

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Salvia miltiorrhiza injection, HPLC, antioxidant activity, correlation analysis, salvianolic acid