<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape- -stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG.</p></abstract>

The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape- -stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG.

In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

Department of Chemical and Pharmaceutical Sciences, University of Ferrara 44121 Ferrara, Italy

Acta Pharmaceutica

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective...

In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

Published Online: Jun 06, 2014

Page range: 257 - 265

Accepted: Feb 07, 2014

DOI: https://doi.org/10.2478/acph-2014-0017

Keywords
(-)-epigallocatechin-3-gallate, topical application, in vivo human study, tape-stripping, emulsion, gel

© by Santo Scalia

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

Published Online: Jun 06, 2014

Page range: 257 - 265

Accepted: Feb 07, 2014

DOI: https://doi.org/10.2478/acph-2014-0017

Keywords(-)-epigallocatechin-3-gallate, topical application, in vivo human study, tape-stripping, emulsion, gel

© by Santo Scalia

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords
(-)-epigallocatechin-3-gallate, topical application, in vivo human study, tape-stripping, emulsion, gel