<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration of 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g<sup>-1</sup>, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.</p></abstract>

The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration of 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g-1, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.

Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

Pharmaceutical Technology Division Department of Chemical Technology Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004 MH, India

Acta Pharmaceutica

The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from...

Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

Published Online: Jul 10, 2013

Page range: 193 - 207

DOI: https://doi.org/10.2478/acph-2013-0020

Keywords
phyllanthin, Phyllanthus amarus (Euphorbiaceae), supercritical carbon dioxide assisted extraction, Box- -Behnken design, HPLC

This content is open access.

Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

Published Online: Jul 10, 2013

Page range: 193 - 207

DOI: https://doi.org/10.2478/acph-2013-0020

Keywordsphyllanthin, Phyllanthus amarus (Euphorbiaceae), supercritical carbon dioxide assisted extraction, Box- -Behnken design, HPLC

This content is open access.

Keywords
phyllanthin, Phyllanthus amarus (Euphorbiaceae), supercritical carbon dioxide assisted extraction, Box- -Behnken design, HPLC