<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.</p></abstract>

The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.

Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue

Institute of Pathology. 1 * Faculty of Medicine. Charles University and General Faculty Hospital in Prague. Czech IP.m.Klin

Acta Medica Martiniana

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely...

Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue

Published Online: May 30, 2014

Page range: 5 - 13

Received: Feb 02, 2014

Accepted: Apr 22, 2014

DOI: https://doi.org/10.2478/acm-2014-0001

Keywords
monoclonality, PCR, IgH, paraffin-embedded tissue, heteroduplex

© by L. Stanek

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue

Published Online: May 30, 2014

Page range: 5 - 13

Received: Feb 02, 2014

Accepted: Apr 22, 2014

DOI: https://doi.org/10.2478/acm-2014-0001

Keywordsmonoclonality, PCR, IgH, paraffin-embedded tissue, heteroduplex

© by L. Stanek

This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords
monoclonality, PCR, IgH, paraffin-embedded tissue, heteroduplex