<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Trophoblast cells can be detected and isolated from the cervical epithelial cells obtained via various techniques of trans-cervical samples collection such as a mucus aspiration, endocervical lavage, or standard cervical brushing in the early first trimester, even from the 5 weeks’ gestation. Isolated fetal cells can be used in the early prediction of fetal sex, prenatal diagnostics of the most common aneuploidies, and any other genetic abnormalities. Nevertheless, the collection of trophoblastic cells has limited efficacy compared to currently used methods of detection of free fetal DNA in maternal circulation or other protocols of invasive prenatal diagnostics available at later stages of pregnancy. In the past years, trans-cervical cell samples were collected mainly in women before planned pregnancy termination. The early trophoblastic cells isolation from women in ongoing pregnancies opens new perspectives for further studies focused on the elucidation of pathophysiology of numerous pregnancy-related complications.</p>
</abstract>

Trophoblast cells can be detected and isolated from the cervical epithelial cells obtained via various techniques of trans-cervical samples collection such as a mucus aspiration, endocervical lavage, or standard cervical brushing in the early first trimester, even from the 5 weeks’ gestation. Isolated fetal cells can be used in the early prediction of fetal sex, prenatal diagnostics of the most common aneuploidies, and any other genetic abnormalities. Nevertheless, the collection of trophoblastic cells has limited efficacy compared to currently used methods of detection of free fetal DNA in maternal circulation or other protocols of invasive prenatal diagnostics available at later stages of pregnancy. In the past years, trans-cervical cell samples were collected mainly in women before planned pregnancy termination. The early trophoblastic cells isolation from women in ongoing pregnancies opens new perspectives for further studies focused on the elucidation of pathophysiology of numerous pregnancy-related complications.

Methods of detection and isolation of trophoblast cells from trans-cervical specimens – a historical overview

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Trophoblast cells can be detected and isolated from the cervical epithelial cells obtained via various techniques of trans-cervical samples collection such as...

Methods of detection and isolation of trophoblast cells from trans-cervical specimens – a historical overview

Published Online: Dec 30, 2021

Page range: 170 - 176

Received: Nov 03, 2021

Accepted: Dec 18, 2021

DOI: https://doi.org/10.2478/acb-2021-0024

Keywords
cervix, pap smear, prenatal diagnostics, trophoblast

© 2021 Rafal Sibiak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Methods of detection and isolation of trophoblast cells from trans-cervical specimens – a historical overview

Published Online: Dec 30, 2021

Page range: 170 - 176

Received: Nov 03, 2021

Accepted: Dec 18, 2021

DOI: https://doi.org/10.2478/acb-2021-0024

Keywordscervix, pap smear, prenatal diagnostics, trophoblast

© 2021 Rafal Sibiak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
cervix, pap smear, prenatal diagnostics, trophoblast