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Telomerase Activity and Myogenesis Ability as an Indicator of Cultured Turkey Satellite Cell Ability for In Vitro Meat Production

Afsaneh Golkar Narenji
Afsaneh Golkar Narenji
, 
James N. Petitte
James N. Petitte
, 
Magdalena Kulus
Magdalena Kulus
, 
Katarzyna Stefańska
Katarzyna Stefańska
, 
Joanna Perek
Joanna Perek
, 
Jakub Kulus
Jakub Kulus
, 
Maria Wieczorkiewicz
Maria Wieczorkiewicz
, 
Maurycy Jankowski
Maurycy Jankowski
, 
Artur Bryja
Artur Bryja
, 
Rut Bryl
Rut Bryl
, 
Paweł Antosik
Paweł Antosik
, 
Dorota Bukowska
Dorota Bukowska
, 
Jędrzej M. Jaśkowki
Jędrzej M. Jaśkowki
, 
Kornel Ratajczak
Kornel Ratajczak
 and 
Paul Mozdziak
Paul Mozdziak
   | Mar 30, 2021
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Published Online: Mar 30, 2021
Page range: 19 - 26
Received: Feb 03, 2021
Accepted: Mar 06, 2021
DOI: https://doi.org/10.2478/acb-2021-0004
Keywords
© 2021 Afsaneh Golkar Narenji et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Detection of telomerase in the cultured cells. A: Primer dimer control, B: Cultured Turkey cells, C*: Heat treated cultured turkey cells, D: Rat myoblast cells, E*: Heat treated rat myoblast cells. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability
Detection of telomerase in the cultured cells. A: Primer dimer control, B: Cultured Turkey cells, C*: Heat treated cultured turkey cells, D: Rat myoblast cells, E*: Heat treated rat myoblast cells. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability

Figure 2

Telomerase detection in undifferentiated versus differentiated cells. A: Primer dimer Control, B: kit positive control, C*: Heat treated kit positive control, D:Undifferentiated cells E*: Heat treated undifferentiated cells, F: Differentiated cells, G*: Heat treated differentiated cells. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability
Telomerase detection in undifferentiated versus differentiated cells. A: Primer dimer Control, B: kit positive control, C*: Heat treated kit positive control, D:Undifferentiated cells E*: Heat treated undifferentiated cells, F: Differentiated cells, G*: Heat treated differentiated cells. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability

Figure 3

Telomerase detection in suspended myoblasts. A:Primer dimer control, B:kit positive control, C: Heat treated kit positive control, D: Short-term turkey cell culture in suspension, E: Heat treated short-term turkey cell culture in suspension, F:Long-term Turkey cell culture in suspension, G: Heat treated Long-term Turkey cell culture in suspension. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability
Telomerase detection in suspended myoblasts. A:Primer dimer control, B:kit positive control, C: Heat treated kit positive control, D: Short-term turkey cell culture in suspension, E: Heat treated short-term turkey cell culture in suspension, F:Long-term Turkey cell culture in suspension, G: Heat treated Long-term Turkey cell culture in suspension. *Samples were treated with 60° C for one hour to inactivate telomerase enzyme. The image is presented as a contrast adjusted negative to ease readability

Figure 4

Gene expression in differentiated versus undifferentiated cells. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as myotube cell versus myoblast cell values
Gene expression in differentiated versus undifferentiated cells. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as myotube cell versus myoblast cell values

Figure 5

Gene expression in long-term suspension culture versus adherent cells. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as long-term suspended culture versus adherent cells FC values
Gene expression in long-term suspension culture versus adherent cells. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as long-term suspended culture versus adherent cells FC values

Figure 6

Gene expression in short-term (3 month) versus long-term (12 month) cell culture in suspension. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as short vs long-term suspended culture cell values
Gene expression in short-term (3 month) versus long-term (12 month) cell culture in suspension. The bar chart shows fold changes of genes measured by quantitative RT-PCR. Mean fold changes are expressed on a log2 scale as short vs long-term suspended culture cell values

Primer sequences were used for the amplification of each gene

Turkey MyoD1Forward: GCTCCAAGACCTGCCTTCTA
Reverse: AAACATTGGCCATTTCCCCG
Turkey MyogeninForward: ACCGCAACCTCCACTCTCTC
Reverse: GCCAGCTCAGTTTTGGACCC
Turkey TERT 1Forward: ACTGCTGAAGACAGTGACGGA
Reverse: AACCTTCATCGCTTAGTCCAGT
Turkey TERT 2Forward: GCTGGCAAGTCTCTGTGACTCTT
Reverse: GGAGAGGGAGCTGCTCAGAAC
Turkey MYF5Forward: AGCTGCCAGTTCTCCCCATC
Reverse: GTGCTCCTCTTCCTCAGGGC
Turkey MRF4Forward: TCCGACTTCCTGAGCACCTG
Reverse: TTTCTCCACCGCCTCTTCCG
Turkey Pax7Forward: TGGCGGATGTGGTTGGGTAA
Reverse: TAGAAGTGTCCACCCCGCTG
Turkey β-actinForward: TACTCCCACAGCCAGCCATG
Reverse: TGGGGTACTTCAGGGTCAGGA
Turkey DyskerinForward: GTTAGCGACTACCCCCGACC
Reverse: GTGGAGACCCCGTTCCTACG
Turkey ALDH1Forward: AATCGCTCCCTTGCTGTTGA
Reverse: AATCGCTCCCTTGCTGTTGA
Turkey ALDH2Forward: GCTAGTCGTAAAGACCCCCG
Reverse: TCTGGCACGGAAAATGCTCA
Turkey NanogForward: CAGAGCAGAAAACGCCCAGC
Reverse: CCTTGTCCCACTCTCACCTTTA
Turkey GAPDHForward: TACTCCCACAGCCAGCCATG
Reverse: ACGGCAGGTCAGGTCAACAA
eISSN:
2544-3577
Language:
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Journal Subjects:
Life Sciences, Molecular Biology, Biochemistry
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