<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of the present study was to assess the suitability of different Amicon Ultra and Centrifree ultrafiltration devices for the study of the plasma protein binding process in the case of carvedilol, a highly protein-bound and lipophilic beta-blocking agent. Samples at different levels of concentration were prepared in both proteic and non-proteic matrices (human plasma, 5% human serum albumin solution and saline solution) and subjected to the classical ultrafiltration method using the different devices considered. Furthermore, an attempt to apply a previously described modified ultrafiltration method was also made. The analysis and quantification was achieved using a validated LC-MS/MS method. For the Centrifree devices, the determined unbound fractions of carvedilol and the corresponding binding degree were in accordance to literature data, while for the Amicon Ultra devices a great degree of carvedilol adsorbtion to the sample reservoir was observed, the analyte not being detected in the ultrafiltrate samples. Thus, it was further demonstrated that the type of ultrafiltration device used has a significant influence on the outcome of a plasma protein binding study. In the case of carvedilol, the evaluation of the protein binding interaction could be achieved using the Centrifree ultrafiltration devices, but not the Amicon Ultra devices.</p>
</abstract>

The aim of the present study was to assess the suitability of different Amicon Ultra and Centrifree ultrafiltration devices for the study of the plasma protein binding process in the case of carvedilol, a highly protein-bound and lipophilic beta-blocking agent. Samples at different levels of concentration were prepared in both proteic and non-proteic matrices (human plasma, 5% human serum albumin solution and saline solution) and subjected to the classical ultrafiltration method using the different devices considered. Furthermore, an attempt to apply a previously described modified ultrafiltration method was also made. The analysis and quantification was achieved using a validated LC-MS/MS method. For the Centrifree devices, the determined unbound fractions of carvedilol and the corresponding binding degree were in accordance to literature data, while for the Amicon Ultra devices a great degree of carvedilol adsorbtion to the sample reservoir was observed, the analyte not being detected in the ultrafiltrate samples. Thus, it was further demonstrated that the type of ultrafiltration device used has a significant influence on the outcome of a plasma protein binding study. In the case of carvedilol, the evaluation of the protein binding interaction could be achieved using the Centrifree ultrafiltration devices, but not the Amicon Ultra devices.

Comparison of Different Ultrafiltration Devices for the Study of Plasma Protein Binding of Carvedilol

I.O.S.U.D., George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Department of Analytical Chemistry and Drug Analysis, Faculty of Pharmacy

George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Acta Biologica Marisiensis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The aim of the present study was to assess the suitability of different Amicon Ultra and Centrifree ultrafiltration devices for the study of the plasma protein...

Comparison of Different Ultrafiltration Devices for the Study of Plasma Protein Binding of Carvedilol

Published Online: Dec 22, 2023

Page range: 1 - 10

Received: Jul 26, 2023

Accepted: Aug 26, 2023

DOI: https://doi.org/10.2478/abmj-2023-0007

Keywords
ultrafiltration, protein binding, carvedilol, Amicon Ultra, Centrifree

© 2023 Camelia-Maria Toma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Comparison of Different Ultrafiltration Devices for the Study of Plasma Protein Binding of Carvedilol

Published Online: Dec 22, 2023

Page range: 1 - 10

Received: Jul 26, 2023

Accepted: Aug 26, 2023

DOI: https://doi.org/10.2478/abmj-2023-0007

Keywordsultrafiltration, protein binding, carvedilol, Amicon Ultra, Centrifree

© 2023 Camelia-Maria Toma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
ultrafiltration, protein binding, carvedilol, Amicon Ultra, Centrifree