<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">Aluminium (Al) exposure may affect the liver of experimental animals. This investigation aimed at evaluating morphological changes in rat liver after a single high dose of Al (as metallic powder suspension). A total of forty female Wistar rats were divided in one exposed and one control group, 20 rats each. The exposed rats received 0.5 mL of sterile physiological suspension of fine Al powder in the concentration of 100 mg mL-1 intraperitoneally (50 mg Al per rat). After 7 weeks all animals were killed (by exsanguination from the abdominal aorta in ether anaesthesia). Liver aluminium was analysed using electrothermal atomic absorption spectrometry. For light microscopy the liver tissue was stained with hematoxylin and eosin, and for histochemical analysis with aurin threecarbocsillic acid (aluminon).Liver Al level was markedly higher in the exposed (37.1 μg g-1) than in control rats (0.71 μg g-1). The exposed rats showed crystalloid Al inclusions in the capsular, subcapsular, and portal liver tissue. The basic liver structure remained intact. Slightly multiplied bile ductuli were found in 16 of 20 exposed and in 8 of 20 control rats. Three exposed rats had mycrovesicular steatosis. The peritoneum and Glisson's capsule showed strong macrophage infiltration and a foreign-body-like reaction with multiple giant macrophages containing Al crystalloid inclusions. Although this reaction was a defense against the metal, some Al passed this barrier and entered the liver tissue, exerting toxic effects in bile ductuli and hepatocytes.</abstract>

Aluminium (Al) exposure may affect the liver of experimental animals. This investigation aimed at evaluating morphological changes in rat liver after a single high dose of Al (as metallic powder suspension). A total of forty female Wistar rats were divided in one exposed and one control group, 20 rats each. The exposed rats received 0.5 mL of sterile physiological suspension of fine Al powder in the concentration of 100 mg mL-1 intraperitoneally (50 mg Al per rat). After 7 weeks all animals were killed (by exsanguination from the abdominal aorta in ether anaesthesia). Liver aluminium was analysed using electrothermal atomic absorption spectrometry. For light microscopy the liver tissue was stained with hematoxylin and eosin, and for histochemical analysis with aurin threecarbocsillic acid (aluminon).Liver Al level was markedly higher in the exposed (37.1 μg g-1) than in control rats (0.71 μg g-1). The exposed rats showed crystalloid Al inclusions in the capsular, subcapsular, and portal liver tissue. The basic liver structure remained intact. Slightly multiplied bile ductuli were found in 16 of 20 exposed and in 8 of 20 control rats. Three exposed rats had mycrovesicular steatosis. The peritoneum and Glisson's capsule showed strong macrophage infiltration and a foreign-body-like reaction with multiple giant macrophages containing Al crystalloid inclusions. Although this reaction was a defense against the metal, some Al passed this barrier and entered the liver tissue, exerting toxic effects in bile ductuli and hepatocytes.

Histopathological Changes in Rat Liver After A Single High Dose of Aluminium

Institute of Occupational Health "Dr Dragomir Karajović", Belgrade, Serbia

University of Belgrade School of Medicine, Belgrade, Serbia

Archives of Industrial Hygiene and Toxicology

{"article-title":"Histopathological Changes in Rat Liver After A Single High Dose of Aluminium"}

Aluminium (Al) exposure may affect the liver of experimental animals. This investigation aimed at evaluating morphological changes in rat liver after a single...

Histopathological Changes in Rat Liver After A Single High Dose of Aluminium

Published Online: Jun 23, 2008

Page range: 97 - 101

DOI: https://doi.org/10.2478/10004-1254-59-2008-1859

Keywords
aluminij, hepatotoksičnost, kristaloidne inkluzije aluminija, laboratorijske štakorice, proliferacija žučnih kanalića

This content is open access.

Histopathological Changes in Rat Liver After A Single High Dose of Aluminium

Published Online: Jun 23, 2008

Page range: 97 - 101

DOI: https://doi.org/10.2478/10004-1254-59-2008-1859

Keywordsaluminij, hepatotoksičnost, kristaloidne inkluzije aluminija, laboratorijske štakorice, proliferacija žučnih kanalića

This content is open access.

Keywords
aluminij, hepatotoksičnost, kristaloidne inkluzije aluminija, laboratorijske štakorice, proliferacija žučnih kanalića