<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in <italic>Escherichia coli</italic> in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further enhancement of the yield, different post-induction periods of time were selected for testing. In all experiments, the productivity level and bacterial cell growth were evaluated by western blotting technique and monitoring of absorbance at 600 nm, respectively. Our results indicated that %DO, the post-induction time and temperature have significant effects on the production of β-NGF. After 2 hours of induction, the low post induction temperature of 32°C and 20% DO were used to increase the production of β-NGF in a 5-l bioreactor. Another important result obtained in this study was that the improved β-NGF production was not achieved at highest dry cell weigh or highest cell growth. These results are definitely of importance for industrial β-NGF production.</p></abstract>

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the optimized conditions for β-NGF overproduction in Escherichia coli in a shake-flask culture. In this study the optimal %DO (dissolved oxygen) and post induction temperature values for improved production of β-NGF were found in the bioreactor scale using response surface methodology (RSM) as the most common statistical method. Also, for further enhancement of the yield, different post-induction periods of time were selected for testing. In all experiments, the productivity level and bacterial cell growth were evaluated by western blotting technique and monitoring of absorbance at 600 nm, respectively. Our results indicated that %DO, the post-induction time and temperature have significant effects on the production of β-NGF. After 2 hours of induction, the low post induction temperature of 32°C and 20% DO were used to increase the production of β-NGF in a 5-l bioreactor. Another important result obtained in this study was that the improved β-NGF production was not achieved at highest dry cell weigh or highest cell growth. These results are definitely of importance for industrial β-NGF production.

Improved Production of Recombinant Human β-NGF in <italic>Escherichia coli</italic> – a Bioreactor Scale Study

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Improved Production of Recombinant Human β-NGF in  – a Bioreactor Scale Study

Human nerve growth factor β (β-NGF) is considered a major therapeutic agent for treatment of neurodegenerative diseases. We have previously reported the...

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Article Category: original-paper

Published Online: Sep 04, 2018

Page range: 355 - 363

Received: Mar 22, 2018

Accepted: Jun 16, 2018

DOI: https://doi.org/10.21307/pjm-2018-045

Keywords
bioreactor, RSM, β-NGF over production,

© 2018 Zahra Hajihassan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Improved Production of Recombinant Human β-NGF in Escherichia coli – a Bioreactor Scale Study

Article Category: original-paper

Published Online: Sep 04, 2018

Page range: 355 - 363

Received: Mar 22, 2018

Accepted: Jun 16, 2018

DOI: https://doi.org/10.21307/pjm-2018-045

Keywordsbioreactor, RSM, β-NGF over production,

© 2018 Zahra Hajihassan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
bioreactor, RSM, β-NGF over production,