<abstract xmlns="http://www.w3.org/1999/xhtml"><p>To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of <italic>Acinetobacter baumannii</italic>, the intensive care unit settings of a hospital in Northern China were surveyed in 2014. Twenty non-duplicate <italic>A. baumannii</italic> isolates were obtained from patients and five isolates of airborne <italic>A. baumannii</italic> were obtained from the wards’ corridors. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homology relationships of isolates. Resistance and resistance genes were detected by drug susceptibility test and PCR. The results demonstrated that all isolates can be classified into eight PFGE types and four sequence types (ST208, ST195, ST369 and ST530). A pair of isolates from patients (TAaba004) and from the air (TAaba012) that share 100% similarity in PFGE was identified, indicating that air might be a potential and important transmission route for <italic>A. baumannii</italic>. More than 80% of the isolates were resistant to carbapenems and aminoglycoside antibiotics. Twenty-four isolates, which were resistant to carbapenems, carried the <italic>bla</italic><sub>OXA-23-like</sub> gene. The data indicated that air might be an alternative way for the transmission of <italic>A. baumannii</italic>. Hospitals should pay more attention to this route, and design new measures accordingly.</p></abstract>

To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of Acinetobacter baumannii, the intensive care unit settings of a hospital in Northern China were surveyed in 2014. Twenty non-duplicate A. baumannii isolates were obtained from patients and five isolates of airborne A. baumannii were obtained from the wards’ corridors. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homology relationships of isolates. Resistance and resistance genes were detected by drug susceptibility test and PCR. The results demonstrated that all isolates can be classified into eight PFGE types and four sequence types (ST208, ST195, ST369 and ST530). A pair of isolates from patients (TAaba004) and from the air (TAaba012) that share 100% similarity in PFGE was identified, indicating that air might be a potential and important transmission route for A. baumannii. More than 80% of the isolates were resistant to carbapenems and aminoglycoside antibiotics. Twenty-four isolates, which were resistant to carbapenems, carried the blaOXA-23-like gene. The data indicated that air might be an alternative way for the transmission of A. baumannii. Hospitals should pay more attention to this route, and design new measures accordingly.

Carbapenem-resistant <italic>Acinetobacter baumannii</italic> from Air and Patients of Intensive Care Units

Polish Journal of Microbiology

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Carbapenem-resistant  from Air and Patients of Intensive Care Units

To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of Acinetobacter baumannii, the intensive care unit settings of...

Antibiotics	Susceptible		Resistant
Antibiotics	Number	Rate (%)	Number	Rate (%)
Amikacin	3	15.0%	17	85.0%
Ceftazidime	0	0.0%	20	100%
Ciprofloxacin	0	0.0%	20	100%
Levofloxacin	0	0.0%	20	100%
Gentamicin	0	0.0%	20	100%
Tobramycin	3	15.0%	17	85.0%
Ceftriaxone	0	0.0%	20	100%
Cefotaxime	0	0.0%	20	100%
Cefepime	0	0.0%	20	100%
Imipenem	0	0.0%	20	100%
Meropenem	0	0.0%	20	100%
Trimethoprim/sulfamethoxazole	6	30.0%	14	100%
Piperacillin/tazobactam	0	0.0%	20	100%
Tigecycline	20	100%	0	0.0%
Polymyxin B	20	100%	0	0.0%

Strain / Antibiotics	TAaba004	TAaba012
Amikacin	R	R
Ceftazidime	R	R
Ciprofloxacin	R	R
Levofloxacin	R	R
Gentamicin	R	R
Tobramycin	R	R
Ceftriaxone	R	R
cefotaxime	R	R
Cefepime	R	R
Imipenem	R	R
Meropenem	R	R
Trimethoprim/sulfamethoxazole	R	R
Piperacillin/tazobactam	R	R
Tigecycline	S	S
Polymyxin B	S	S

Carbapenem-resistant Acinetobacter baumannii from Air and Patients of Intensive Care Units

Article Category: original-paper

Published Online: Sep 04, 2018

Page range: 333 - 338

Received: Oct 28, 2017

Accepted: Jul 07, 2018

DOI: https://doi.org/10.21307/pjm-2018-040

Keywords
molecular epidemiology, antibiotic resistance, airborne, nosocomial infection

© 2018 Meijie Jiang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Susceptibility profiles of 20 clinical A. baumannii isolates.

Susceptibility profiles of the airborne and clinical strains with the highest similarity.

Carbapenem-resistant Acinetobacter baumannii from Air and Patients of Intensive Care Units

Article Category: original-paper

Published Online: Sep 04, 2018

Page range: 333 - 338

Received: Oct 28, 2017

Accepted: Jul 07, 2018

DOI: https://doi.org/10.21307/pjm-2018-040

Keywordsmolecular epidemiology, antibiotic resistance, airborne, nosocomial infection

© 2018 Meijie Jiang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Susceptibility profiles of 20 clinical A. baumannii isolates.

Susceptibility profiles of the airborne and clinical strains with the highest similarity.

Keywords
molecular epidemiology, antibiotic resistance, airborne, nosocomial infection