<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The application of DNA-based methods enables to identify <italic>Yersinia enterocolitica</italic> carrying the <italic>ail</italic>-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic <italic>Y. enterocolitica</italic> in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the <italic>ail</italic> gene. Additionally, biochemical tests and RT-PCR showed that nine <italic>Y. enterocolitica</italic> isolates carried the <italic>ail</italic>-gene. Two <italic>Y. enterocolitica</italic> isolates of 1A biotype had the <italic>ail</italic> gene. The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the <italic>ail</italic> gene <italic>Y. enterocolitica</italic> in the material examined.</p></abstract>

The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic Y. enterocolitica in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the ail gene. Additionally, biochemical tests and RT-PCR showed that nine Y. enterocolitica isolates carried the ail-gene. Two Y. enterocolitica isolates of 1A biotype had the ail gene. The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the ail gene Y. enterocolitica in the material examined.

Identification of Pathogenicity of <italic>Yersinia enterocolitica</italic> in Pig Tonsils Using the Real-Time PCR

Polish Journal of Microbiology

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Identification of Pathogenicity of  in Pig Tonsils Using the Real-Time PCR

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The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods...

Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

Article Category: short-communication

Published Online: Jun 30, 2018

Page range: 219 - 222

Received: Jun 06, 2017

Accepted: Feb 22, 2018

DOI: https://doi.org/10.21307/pjm-2018-023

Keywords
RT-PCR, pathogen specific gene (), biochemical testing, biotyping

© 2018 Milena A. Stachelska published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

Article Category: short-communication

Published Online: Jun 30, 2018

Page range: 219 - 222

Received: Jun 06, 2017

Accepted: Feb 22, 2018

DOI: https://doi.org/10.21307/pjm-2018-023

KeywordsRT-PCR, pathogen specific gene (), biochemical testing, biotyping

© 2018 Milena A. Stachelska published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
RT-PCR, pathogen specific gene (), biochemical testing, biotyping