<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A total of 123 <italic>Trichoderma</italic> strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening method. Among strains, more than 34 and 45% respectively, exhibited higher cellulolytic and xylanolytic activity, compared to the reference strain <italic>T. reesei</italic> QM 9414. For strains efficiently degrading cellulose, a highest enzyme activity was confirmed using filter paper test, and it resulted in a range from 1.01 to 7.15 FPU/ml. Based on morphological and molecular analysis, the isolates were identified as <italic>Trichoderma</italic>. The most frequently identified strains belonged to <italic>Trichoderma harzianum</italic> species. Among all strains, the most effective in degradation of cellulose and xylose was <italic>T. harzianum</italic> and <italic>T. virens</italic>, especially those isolated from forest wood, forest soil or garden and mushroom compost. The results of this work confirmed that numerous strains from the <italic>Trichoderma</italic> species have high cellulose and xylan degradation potential and could be useful for lignocellulose biomass conversion <italic>e.g</italic>. for biofuel production.</p></abstract>

A total of 123 Trichoderma strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening method. Among strains, more than 34 and 45% respectively, exhibited higher cellulolytic and xylanolytic activity, compared to the reference strain T. reesei QM 9414. For strains efficiently degrading cellulose, a highest enzyme activity was confirmed using filter paper test, and it resulted in a range from 1.01 to 7.15 FPU/ml. Based on morphological and molecular analysis, the isolates were identified as Trichoderma. The most frequently identified strains belonged to Trichoderma harzianum species. Among all strains, the most effective in degradation of cellulose and xylose was T. harzianum and T. virens, especially those isolated from forest wood, forest soil or garden and mushroom compost. The results of this work confirmed that numerous strains from the Trichoderma species have high cellulose and xylan degradation potential and could be useful for lignocellulose biomass conversion e.g. for biofuel production.

Screening and Identification of <italic>Trichoderma</italic> Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Screening and Identification of  Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

A total of 123 Trichoderma strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening...

Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

Article Category: original-paper

Published Online: Jun 30, 2018

Page range: 181 - 190

Received: May 22, 2017

Accepted: Feb 02, 2018

DOI: https://doi.org/10.21307/pjm-2018-021

Keywords
microorganisms screening, species, lignocellulose biomass, cellulolytic activity, xylanolytic activity

© 2018 ROMAN MARECIK et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

Article Category: original-paper

Published Online: Jun 30, 2018

Page range: 181 - 190

Received: May 22, 2017

Accepted: Feb 02, 2018

DOI: https://doi.org/10.21307/pjm-2018-021

Keywordsmicroorganisms screening, species, lignocellulose biomass, cellulolytic activity, xylanolytic activity

© 2018 ROMAN MARECIK et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
microorganisms screening, species, lignocellulose biomass, cellulolytic activity, xylanolytic activity