<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>
<italic>Meloidogyne chitwoodi</italic> is a root-knot nematode that is a major pest of potato in the northwestern United States. Due to the lack of resistance against root-knot nematodes in potato, research has been undertaken to understand the <italic>M. chitwoodi</italic>-potato interaction at the molecular level. To identify the nematode genes that are playing roles in parasitism, we have performed transcriptome analyses on pre-parasitic and parasitic <italic>M. chitwoodi</italic> juveniles in susceptible potato. We compared gene expression profiles and identified genes that were significantly up- or down- regulated during nematode parasitism. Because parasitism proteins are typically secreted by the nematode to facilitate infection of host roots, we focused on the genes that encoded proteins that were predicted to be secreted. We found that approximately 34% (43/127) of the genes in the predicted secretome encoded proteins with no significant homology in the public genome databases, and 12% (15/127) encoded either a known effector, putative effectors or putative esophageal gland cell proteins. The transcriptome analyses of <italic>M. chitwoodi</italic> at the pre-parasitic and parasitic life stages shed light on the genes involved in nematode parasitism.</p>
</abstract>

Meloidogyne chitwoodi is a root-knot nematode that is a major pest of potato in the northwestern United States. Due to the lack of resistance against root-knot nematodes in potato, research has been undertaken to understand the M. chitwoodi-potato interaction at the molecular level. To identify the nematode genes that are playing roles in parasitism, we have performed transcriptome analyses on pre-parasitic and parasitic M. chitwoodi juveniles in susceptible potato. We compared gene expression profiles and identified genes that were significantly up- or down- regulated during nematode parasitism. Because parasitism proteins are typically secreted by the nematode to facilitate infection of host roots, we focused on the genes that encoded proteins that were predicted to be secreted. We found that approximately 34% (43/127) of the genes in the predicted secretome encoded proteins with no significant homology in the public genome databases, and 12% (15/127) encoded either a known effector, putative effectors or putative esophageal gland cell proteins. The transcriptome analyses of M. chitwoodi at the pre-parasitic and parasitic life stages shed light on the genes involved in nematode parasitism.

<article-title xml:lang="en">Transcriptome Analyses of Pre-parasitic and Parasitic <italic>Meloidogyne Chitwoodi</italic> Race 1 to Identify Putative Effector Genes

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Transcriptome Analyses of Pre-parasitic and Parasitic  Race 1 to Identify Putative Effector Genes

Meloidogyne chitwoodi is a root-knot nematode that is a major pest of potato in the northwestern United States. Due to the lack of resistance against root-knot...

Transcriptome Analyses of Pre-parasitic and Parasitic Meloidogyne Chitwoodi Race 1 to Identify Putative Effector Genes

Published Online: Oct 12, 2021

Page range: 1 - 13

DOI: https://doi.org/10.21307/jofnem-2021-084

Keywords
Differentially expressed genes, Host-parasitic relationship, Meloidogyne, Molecular biology, Transcriptome

© 2021 Lei Zhang et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Transcriptome Analyses of Pre-parasitic and Parasitic Meloidogyne Chitwoodi Race 1 to Identify Putative Effector Genes

Published Online: Oct 12, 2021

Page range: 1 - 13

DOI: https://doi.org/10.21307/jofnem-2021-084

KeywordsDifferentially expressed genes, Host-parasitic relationship, Meloidogyne, Molecular biology, Transcriptome

© 2021 Lei Zhang et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Differentially expressed genes, Host-parasitic relationship, Meloidogyne, Molecular biology, Transcriptome