<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>
<italic>Meloidogyne incognita</italic> is an obligate plant-parasitic nematode causing serious damage to agricultural crops. Major constraints in nematode management arose due to the limited availability of non-fumigant nematicides in conjunction with the considerable ill effects of fumigants on human and non-target organisms. Recently, fluensulfone has been reported to be an effective non-fumigant nematicide against plant-parasitic nematodes and the model nematode <italic>Caenorhabditis elegans.</italic> The nematicidal efficacy varies according to its concentration at the time of application, exposure timing, nematode species variability, and even across subpopulations within the same species. It interferes with the key physiological processes of nematodes, like motility, behavior, chemosensation, stylet thrusting, infectivity, metabolism, lipid consumption, tissue integrity, oviposition, egg hatching, and survival. However, the molecular basis of these multivariate physiological anomalies is still largely unknown. Quantitative real-time PCR was carried out to understand the acute transcriptional perturbation of 30 functional genes associated with key physiological and life processes in a <italic>M. incognita</italic> population, following exposure of 10, 50, and 100 ppm of fluensulfone for 5 and 10 hr. The chemical treatment resulted in significant downregulation of all the neuropeptidergic genes, with concomitant repression of majority of genes related to chemosensation, esophageal gland secretion, parasitism, fatty acid metabolism, and G-protein coupled receptors. Collectively, the parasitism genes were found to be perturbed at highest magnitude, followed by the GPCRs and neuropeptidergic genes. These results establish the wide ranging effect of fluensulfone on various metabolic and physiological pathways of nematode.</p>
</abstract>

Meloidogyne incognita is an obligate plant-parasitic nematode causing serious damage to agricultural crops. Major constraints in nematode management arose due to the limited availability of non-fumigant nematicides in conjunction with the considerable ill effects of fumigants on human and non-target organisms. Recently, fluensulfone has been reported to be an effective non-fumigant nematicide against plant-parasitic nematodes and the model nematode Caenorhabditis elegans. The nematicidal efficacy varies according to its concentration at the time of application, exposure timing, nematode species variability, and even across subpopulations within the same species. It interferes with the key physiological processes of nematodes, like motility, behavior, chemosensation, stylet thrusting, infectivity, metabolism, lipid consumption, tissue integrity, oviposition, egg hatching, and survival. However, the molecular basis of these multivariate physiological anomalies is still largely unknown. Quantitative real-time PCR was carried out to understand the acute transcriptional perturbation of 30 functional genes associated with key physiological and life processes in a M. incognita population, following exposure of 10, 50, and 100 ppm of fluensulfone for 5 and 10 hr. The chemical treatment resulted in significant downregulation of all the neuropeptidergic genes, with concomitant repression of majority of genes related to chemosensation, esophageal gland secretion, parasitism, fatty acid metabolism, and G-protein coupled receptors. Collectively, the parasitism genes were found to be perturbed at highest magnitude, followed by the GPCRs and neuropeptidergic genes. These results establish the wide ranging effect of fluensulfone on various metabolic and physiological pathways of nematode.

<article-title xml:lang="en">Effect of fluensulfone on different functional genes of root-knot nematode <italic>Meloidogyne incognita</italic>


Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of fluensulfone on different functional genes of root-knot nematode 


Meloidogyne incognita is an obligate plant-parasitic nematode causing serious damage to agricultural crops. Major constraints in nematode management arose due...

Effect of fluensulfone on different functional genes of root-knot nematode Meloidogyne incognita

Published Online: Aug 17, 2021

Page range: 1 - 14

DOI: https://doi.org/10.21307/jofnem-2021-073

Keywords
Chemosensation, Fluensulfone, Metabolism, Neurotransmission, Parasitism, Root-knot nematode

© 2021 Alkesh Hada et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of fluensulfone on different functional genes of root-knot nematode Meloidogyne incognita

Published Online: Aug 17, 2021

Page range: 1 - 14

DOI: https://doi.org/10.21307/jofnem-2021-073

KeywordsChemosensation, Fluensulfone, Metabolism, Neurotransmission, Parasitism, Root-knot nematode

© 2021 Alkesh Hada et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Chemosensation, Fluensulfone, Metabolism, Neurotransmission, Parasitism, Root-knot nematode