<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Alive anisakids cause acute gastrointestinal diseases, and dead worms contained in food can provoke sensibilization and allergic reactions in humans. Detected in the purchased minced salmon <italic>Oncorhynchus nerka</italic> nematodes were identified as <italic>Anisakis simplex sensu stricto</italic> (Anisakidae). We found that recently published phylogenetic trees (reconstructed using different ribosomal and mitochondrial genetic markers) showed independent clusterization of species recognized in the <italic>A. simplex sensu lato</italic> species complex. This prompted us to undertake this full-fledged molecular genetics study of anisakids from Kamchatka with phylogenetic reconstructions (NJ/ML) and calculated ranges of interspecific and intergeneric p-distances using ITS1-5.8S-ITS2 sequences. We confirmed that molecular markers based on the ITS region of rDNA were able to recognize ‘pure’ specimens belonging to the cryptic species. We offer new insights into the systematics of anisakids. The genus <italic>Anisakis sensu stricto</italic> should include <italic>Anisakis simplex sensu stricto</italic>, <italic>Anisakis pegreffii</italic>, <italic>Anisakis berlandi</italic>, <italic>Anisakis ziphidarum</italic>, and <italic>Anisakis nascettii</italic>. Presumably, two genera should be restored in the structure of the subfamily Anisakinae: <italic>Skrjabinisakis</italic> for the species <italic>Anisakis paggiae</italic>, <italic>Anisakis brevispiculata</italic>, and <italic>Anisakis physeteris</italic>; and <italic>Peritrachelius</italic> for the species <italic>Anisakis typica</italic>. In addition, we provide the short annotated list of some genera of the family Anisakidae, including their diagnoses.</p>
</abstract>

Alive anisakids cause acute gastrointestinal diseases, and dead worms contained in food can provoke sensibilization and allergic reactions in humans. Detected in the purchased minced salmon Oncorhynchus nerka nematodes were identified as Anisakis simplex sensu stricto (Anisakidae). We found that recently published phylogenetic trees (reconstructed using different ribosomal and mitochondrial genetic markers) showed independent clusterization of species recognized in the A. simplex sensu lato species complex. This prompted us to undertake this full-fledged molecular genetics study of anisakids from Kamchatka with phylogenetic reconstructions (NJ/ML) and calculated ranges of interspecific and intergeneric p-distances using ITS1-5.8S-ITS2 sequences. We confirmed that molecular markers based on the ITS region of rDNA were able to recognize ‘pure’ specimens belonging to the cryptic species. We offer new insights into the systematics of anisakids. The genus Anisakis sensu stricto should include Anisakis simplex sensu stricto, Anisakis pegreffii, Anisakis berlandi, Anisakis ziphidarum, and Anisakis nascettii. Presumably, two genera should be restored in the structure of the subfamily Anisakinae: Skrjabinisakis for the species Anisakis paggiae, Anisakis brevispiculata, and Anisakis physeteris; and Peritrachelius for the species Anisakis typica. In addition, we provide the short annotated list of some genera of the family Anisakidae, including their diagnoses.

<article-title xml:lang="en">First report on molecular identification of <italic>Anisakis simplex</italic> in <italic>Oncorhynchus nerka</italic> from the fish market, with taxonomical issues within Anisakidae

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

First report on molecular identification of  in  from the fish market, with taxonomical issues within Anisakidae

Alive anisakids cause acute gastrointestinal diseases, and dead worms contained in food can provoke sensibilization and allergic reactions in humans. Detected...

First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

Published Online: Jan 01, 2021

Page range: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-023

Keywords
Detection, Anisakidosis, Systematics, Taxonomy, ITS,

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae

Published Online: Jan 01, 2021

Page range: 1 - 10

DOI: https://doi.org/10.21307/jofnem-2021-023

KeywordsDetection, Anisakidosis, Systematics, Taxonomy, ITS,

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Detection, Anisakidosis, Systematics, Taxonomy, ITS,