<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The microbial loop has been suggested as an alternative route for better utilization of phytate, a poorly available P source to plants. We hypothesized that bacterial grazer activity might dramatically enhance bacterial migration and proliferation, increasing the probability of phytate hydrolysis by bacterial phytases and, thus, phytate mineralization and release of free phosphate. We tested this hypothesis in a two-compartment system with a solid medium containing phytate or free phosphate as the source of P. Two bacterial species, <italic>B. subtilis</italic> 168 or <italic>Bradyrhizobium</italic> sp., with or without bacterial grazing nematodes belonging to <italic>Acrobeloides</italic> sp. previously fed on each of the bacterial species, were inoculated at a single point in the medium. Whatever the P source, nematode migration within both zones allowed the proliferation of bacteria. However, <italic>B. subtilis</italic> 168 was more efficient in using phytate than <italic>Bradyrhizobium</italic> sp. since the highest bacterial cell density and free phosphate concentrations were reached by <italic>Acrobeloides</italic> sp. fed on <italic>B. subtilis</italic> 168. The grazer activity seemed to be crucial to enhance phytate mineralization, despite <italic>Acrobeloides</italic> sp. showing a higher preference to feed on <italic>Bradyrhizobium</italic> sp. This study provides new insights into the effects of bacterial grazer activity on phytate mineralization.</p>
</abstract>

The microbial loop has been suggested as an alternative route for better utilization of phytate, a poorly available P source to plants. We hypothesized that bacterial grazer activity might dramatically enhance bacterial migration and proliferation, increasing the probability of phytate hydrolysis by bacterial phytases and, thus, phytate mineralization and release of free phosphate. We tested this hypothesis in a two-compartment system with a solid medium containing phytate or free phosphate as the source of P. Two bacterial species, B. subtilis 168 or Bradyrhizobium sp., with or without bacterial grazing nematodes belonging to Acrobeloides sp. previously fed on each of the bacterial species, were inoculated at a single point in the medium. Whatever the P source, nematode migration within both zones allowed the proliferation of bacteria. However, B. subtilis 168 was more efficient in using phytate than Bradyrhizobium sp. since the highest bacterial cell density and free phosphate concentrations were reached by Acrobeloides sp. fed on B. subtilis 168. The grazer activity seemed to be crucial to enhance phytate mineralization, despite Acrobeloides sp. showing a higher preference to feed on Bradyrhizobium sp. This study provides new insights into the effects of bacterial grazer activity on phytate mineralization.

<article-title xml:lang="en">The grazing activity of <italic>Acrobeloides</italic> sp. drives phytate mineralisation within its trophic relationship with bacteria

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The grazing activity of  sp. drives phytate mineralisation within its trophic relationship with bacteria

The microbial loop has been suggested as an alternative route for better utilization of phytate, a poorly available P source to plants. We hypothesized that...

The grazing activity of Acrobeloides sp. drives phytate mineralisation within its trophic relationship with bacteria

Published Online: Jan 01, 2021

Page range: 1 - 13

DOI: https://doi.org/10.21307/jofnem-2021-021

Keywords
sp, Bacterial proliferation, Nematode migration, Micro-food web interactions, Phytase-producing bacteria, Phosphorus cycling

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The grazing activity of Acrobeloides sp. drives phytate mineralisation within its trophic relationship with bacteria

Published Online: Jan 01, 2021

Page range: 1 - 13

DOI: https://doi.org/10.21307/jofnem-2021-021

Keywordssp, Bacterial proliferation, Nematode migration, Micro-food web interactions, Phytase-producing bacteria, Phosphorus cycling

© 2021 Authors, published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
sp, Bacterial proliferation, Nematode migration, Micro-food web interactions, Phytase-producing bacteria, Phosphorus cycling