<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Root-knot nematode, <italic>Meloidogyne mali</italic> can cause damage in trees, shrubs, and herbaceous plants, and was placed on the EPPO Alert List in 2014. In the present study, we report a population isolated from Japanese maple. The recovered population is described by detailed morphological and molecular approaches. The molecular phylogentic analysis based on 28S rRNA, ITS, and mitochondrial COI genes places the population in the clade together with other <italic>M. mali</italic> sequences available in GenBank. The cloned sequences of the 28S rRNA gene revealed a high intragenomic rRNA polymorphism where the polymorphic copies are spread across <italic>M. mali</italic> clade. Similarly, we also found high variation in the mitochondrial COI gene. Among four haplotypes in <italic>M. mali</italic>, three occur in the newly found population. Our study provides the first report of intragenomic polymorphism in <italic>M. mali</italic>, and the results suggest that intragenomic polymorphism maybe widespread in <italic>Meloidogyne.</italic>
</p>
</abstract>

Root-knot nematode, Meloidogyne mali can cause damage in trees, shrubs, and herbaceous plants, and was placed on the EPPO Alert List in 2014. In the present study, we report a population isolated from Japanese maple. The recovered population is described by detailed morphological and molecular approaches. The molecular phylogentic analysis based on 28S rRNA, ITS, and mitochondrial COI genes places the population in the clade together with other M. mali sequences available in GenBank. The cloned sequences of the 28S rRNA gene revealed a high intragenomic rRNA polymorphism where the polymorphic copies are spread across M. mali clade. Similarly, we also found high variation in the mitochondrial COI gene. Among four haplotypes in M. mali, three occur in the newly found population. Our study provides the first report of intragenomic polymorphism in M. mali, and the results suggest that intragenomic polymorphism maybe widespread in Meloidogyne.

Morphological and molecular analyses of a <italic>Meloidogyne mali</italic> population with high intragenomic rRNA polymorphism

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological and molecular analyses of a  population with high intragenomic rRNA polymorphism

right-running

Root-knot nematode, Meloidogyne mali can cause damage in trees, shrubs, and herbaceous plants, and was placed on the EPPO Alert List in 2014. In the present...

Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

Published Online: Nov 06, 2020

Page range: 1 - 11

DOI: https://doi.org/10.21307/jofnem-2020-105

Keywords
Haplotype, Japanese maple, Mitochondrial COI, Phylogeny, Root-knot nematode

© 2020 Jianfeng Gu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

Published Online: Nov 06, 2020

Page range: 1 - 11

DOI: https://doi.org/10.21307/jofnem-2020-105

KeywordsHaplotype, Japanese maple, Mitochondrial COI, Phylogeny, Root-knot nematode

© 2020 Jianfeng Gu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Haplotype, Japanese maple, Mitochondrial COI, Phylogeny, Root-knot nematode