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Aphelenchus yinyuensis n. sp. (Tylenchina: Aphelenchoididae) found in Terminalia sp. in China

Gu Jianfeng
Gu Jianfeng
, 
Munawar Maria
Munawar Maria
, 
Yiwu Fang
Yiwu Fang
, 
Liu Lele
Liu Lele
, 
Xianfeng Chen
Xianfeng Chen
 and 
Bo Cai
Bo Cai
   | Sep 03, 2020
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Published Online: Sep 03, 2020
Page range: 1 - 12
DOI: https://doi.org/10.21307/jofnem-2020-085
Keywords
© 2020 Gu Jianfeng et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region; D: Lip region; E: Vulval region lateral view; F: Vulval region ventral view; G: Female reproductive system; H, I: Female tails; J: Male tail. Scale bars (A, B = 20 μm; C-J = 10 μm).
Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region; D: Lip region; E: Vulval region lateral view; F: Vulval region ventral view; G: Female reproductive system; H, I: Female tails; J: Male tail. Scale bars (A, B = 20 μm; C-J = 10 μm).

Figure 2:

Light photomicrographs of Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region: D: Lip region; E: Lateral lines; F: Vulval region lateral view; G: Vulval region ventral view; H: Vulval region with post-uterine sac; I: Female reproductive system; J, K: Female tails; L, M: Male tail showing papillae arrangement (arrowheads). (ex = excretory pore; h = hemizonid; P + number = genital papillae; scale bars = A, B = 20 μm; C-M = 10 μm).
Light photomicrographs of Aphelenchus yinyuensis n. sp. A: Entire male; B: Entire female; C: Anterior region: D: Lip region; E: Lateral lines; F: Vulval region lateral view; G: Vulval region ventral view; H: Vulval region with post-uterine sac; I: Female reproductive system; J, K: Female tails; L, M: Male tail showing papillae arrangement (arrowheads). (ex = excretory pore; h = hemizonid; P + number = genital papillae; scale bars = A, B = 20 μm; C-M = 10 μm).

Figure 3:

Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on near full length 18S of rDNA. Bayesian tree inferred from 18S under GTR + I + G model (lnL = −19,759.9398; freqA = 0.2462; freqC = 0.2010; freqG = 0.2710; freqT = 0.2818; R(a) = 1.1079; R(b) = 2.1894; R(c) = 1.2504; R(d) = 0.7956; R(e) = 3.9256; R(f) = 1.0000; Pinvar = 0.1260; Shape = 0.5890). Posterior probability values exceeding 50% are given on appropriate clades.
Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on near full length 18S of rDNA. Bayesian tree inferred from 18S under GTR + I + G model (lnL = −19,759.9398; freqA = 0.2462; freqC = 0.2010; freqG = 0.2710; freqT = 0.2818; R(a) = 1.1079; R(b) = 2.1894; R(c) = 1.2504; R(d) = 0.7956; R(e) = 3.9256; R(f) = 1.0000; Pinvar = 0.1260; Shape = 0.5890). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 4:

Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on 28S D2 to D3 of rDNA. Bayesian tree inferred from 28S D2 to D3 under GTR + I + G model (lnL = −17,543.4883; freqA = 0.1670; freqC = 0.1883; freqG = 0.3314; freqT = 0.3134; R(a) = 1.3541; R(b) = 3.5721; R(c) = 1.7006; R(d) = 0.5742; R(e) = 4.6218; R(f) = 1.0000; Pinvar = 0.1400; Shape = 0.6940). Posterior probability values exceeding 50% are given on appropriate clades.
Phylogenetic relationship of Aphelenchus yinyuensis n. sp. and other nematodes based on 28S D2 to D3 of rDNA. Bayesian tree inferred from 28S D2 to D3 under GTR + I + G model (lnL = −17,543.4883; freqA = 0.1670; freqC = 0.1883; freqG = 0.3314; freqT = 0.3134; R(a) = 1.3541; R(b) = 3.5721; R(c) = 1.7006; R(d) = 0.5742; R(e) = 4.6218; R(f) = 1.0000; Pinvar = 0.1400; Shape = 0.6940). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics of Aphelenchus yinyuensis n. sp.

Female Male
Character Holotype Paratypes Paratypes
n – 15 15
L 792 793 ± 71.9 (639-877) 756 ± 59.6 (647-863)
a 31.0 30.5 ± 2.7 (26.9-33.6) 31.9 ± 3.4 (26.7-37.0)
b 9.6 7.7 ± 1.0 (6.2-8.8) 7.5 ± 0.7 (6.1-8.7)
b′ 5.0 4.8 ± 0.6 (3.9-5.7) 4.5 ± 0.4 (3.8-5.3)
c 19.5 21.0 ± 3.1 (16.7-27.1) 26.7 ± 2.6 (22.1-30.4)
c′ 3.1 2.7 ± 0.4 (2.3-3.8) 1.8 ± 0.2 (1.5-2.2)
V or T 72.7 72.8 ± 3.8 (65.2-77.6) 70.5 ± 7.1 (59.4-89.0)
Lip region height 3.1 3.6 ± 0.3 (3.1-4.2) 3.4 ± 0.5 (2.5-4.2)
Lip region width 8.3 8.8 ± 0.5 (8.1-9.5) 8.5 ± 0.5 (7.8-9.5)
Stylet length 13.7 14.3 ± 0.7 (13.2-15.2) 13.8 ± 1.0 (12.2-15.7)
Body diam. 25.5 26.2 ± 3.1 (19.2-31.7) 23.9 ± 2.1 (19.2-28.2)
Median bulb width 15.3 16.1 ± 1.5 (13.1-19.6) 13.8 ± 1.0 (12.2-15.7)
Median bulb length 22.5 22.3 ± 1.8 (18.6-24.5) 20.1 ± 1.2 (17.8-21.9)
Median bulb length/diam. Ratio 1.5 1.4 ± 0.1 (1.2-1.5) 1.5 ± 0.1 (1.3-1.7)
E. pore from anterior end 113.6 107.9 ± 7.2 (96.6-123.3) 106.5 ± 7.2 (91.5-116.7)
Ovary length or Testis 436.3 407.4 ± 50.2 (280.2-458.3) 531.4 ± 54.1 (452.6-610.9)
Post-uterine sac 112.1 100.7 ± 15.5 (66.3-120.2) –
Vulva to anus distance 176.3 166.9 ± 22.3 (131.2-195.0) –
Post-uterine sac length/vulva to anus (%) 63.6 60.9 ± 10.4 (44.5-82.7) –
Anal (cloacal) body diameter 13.1 14.2 ± 2.0 (10.6-17.3) 15.4 ± 1.0 (13.9-17.3)
Tail length 40.6 38.4 ± 5.9 (24.2-44.9) 28.4 ± 2.4 (23.6-32.2)
Spicule (curved median line) – – 28.0 ± 2.0 (24.3-31.3)
Spicule (Chord) – – 28.7 ± 1.7 (25.8-32.3)
Gubernaculum – – 13.2 ± 0.4 (12.2-13.7)
Hemizonid 115.3 110.1 ± 5.5 (100.1-117.3) 108.5 ± 7.3 (96.1-121.3)
Head to pharyngo-intestinal junction 92.9 103.2 ± 6.9 (91.0-113.5) 100.6 ± 5.5 (86.0-109.3)
Head to pharyngeal gland end 159.6 163.9 ± 12.1 (139.6-181.0) 165.9 ± 12.0 (149.3-188.2)
Vulva to tail terminus 203.1 213.1 ± 28.6 (156.3-251.8) –
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