<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>A survey of entomopathogenic nematodes was conducted in sugar cane crops in a total of 14 soils, and positive results were obtained for strain MC5-2014 in the municipality of Tepalcingo, Morelos, in soil with a sandy loam texture and a pH of 6.4. Species determination was performed via morphological and morphometric techniques by searching for a tubular stoma with a swollen cylindrical pharyngeal body and a metacorpus in the basal part. The range of body length (<italic>L</italic>) was 750 to 1200 μm in females and 720 to 910 μm in males, while the corresponding maximum widths (<italic>W</italic>) of the body were 30 to 60 μm and 20 to 30 μm, respectively. Males exhibited bursa with a 1 + 1 + 3 + 3 distribution of papillae, and females exhibited a vulva located at the mid-body. For molecular identification, the ITS region of ribosomal DNA was used. Virulence tests (LC<sub>50</sub>) were conducted with <italic>Galleria mellonella</italic>, and a value of 4.732 was obtained for infective juveniles (IJs). Taking taxonomic and molecular characteristics into account, the isolate was determined to be <italic>Oscheius myriophila</italic>. The isolation of this strain represents the first geographic report of <italic>O. myriophila</italic> in Mexico, and it should be noted that the cultivation of sugar cane occurs with constant application of insecticides, herbicides, fungicides, and fertilizers as well as harvesting activities such as burning of the crop for harvest. The <italic>O. myriophila</italic> isolate has the potential to be used in the future as a method of biological control in our country.</p>
</abstract>

A survey of entomopathogenic nematodes was conducted in sugar cane crops in a total of 14 soils, and positive results were obtained for strain MC5-2014 in the municipality of Tepalcingo, Morelos, in soil with a sandy loam texture and a pH of 6.4. Species determination was performed via morphological and morphometric techniques by searching for a tubular stoma with a swollen cylindrical pharyngeal body and a metacorpus in the basal part. The range of body length (L) was 750 to 1200 μm in females and 720 to 910 μm in males, while the corresponding maximum widths (W) of the body were 30 to 60 μm and 20 to 30 μm, respectively. Males exhibited bursa with a 1 + 1 + 3 + 3 distribution of papillae, and females exhibited a vulva located at the mid-body. For molecular identification, the ITS region of ribosomal DNA was used. Virulence tests (LC50) were conducted with Galleria mellonella, and a value of 4.732 was obtained for infective juveniles (IJs). Taking taxonomic and molecular characteristics into account, the isolate was determined to be Oscheius myriophila. The isolation of this strain represents the first geographic report of O. myriophila in Mexico, and it should be noted that the cultivation of sugar cane occurs with constant application of insecticides, herbicides, fungicides, and fertilizers as well as harvesting activities such as burning of the crop for harvest. The O. myriophila isolate has the potential to be used in the future as a method of biological control in our country.


<italic>Oscheius myriophila</italic> (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.


 (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

A survey of entomopathogenic nematodes was conducted in sugar cane crops in a total of 14 soils, and positive results were obtained for strain MC5-2014 in the...

Oscheius myriophila (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

Published Online: Jul 28, 2020

Page range: 1 - 8

DOI: https://doi.org/10.21307/jofnem-2020-073

Keywords
First report, México, sugar cane, virulence

© 2020 Juan Manuel Caspeta-Mandujano et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Oscheius myriophila (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

Published Online: Jul 28, 2020

Page range: 1 - 8

DOI: https://doi.org/10.21307/jofnem-2020-073

KeywordsFirst report, México, sugar cane, virulence

© 2020 Juan Manuel Caspeta-Mandujano et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
First report, México, sugar cane, virulence