<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Morphological and molecular analyses of plant<bold>-</bold>parasitic nematodes (PPN) from 12 sugarcane plantation sites of Tanganyika Planting Company (TPC) Limited in Kilimanjaro region of Tanzania revealed the presence of six PPN genera, i.e. <italic>Helicotylenchus</italic>, <italic>Hemicycliophora</italic>, <italic>Pratylenchus</italic>, <italic>Rotylenchulus</italic>, <italic>Scutellonema</italic>, and <italic>Tylenchorhynchus</italic>. The genera with the highest densities and present in virtually all samples were <italic>Pratylenchus</italic> and <italic>Rotylenchulus</italic>, and the most important species appeared to be <italic>R. parvus</italic>, <italic>P. zeae</italic>, <italic>T. crassicaudatus</italic>, and <italic>T. ventrosignatus</italic>. A total sequences of 11 partial ITS, 15 D2-D3 of 28S, and 6 partial 18S of rRNA gene, and 7 partial <italic>COI</italic> gene of mtDNA of these species were obtained in this study. Morphology and molecular data comparisons between the Tanzanian <italic>R. parvus</italic> and the South African <italic>R. parvus</italic> indicated that <italic>R. parvus</italic> is a cryptic species complex. Based on the results of morphological and molecular analyses of <italic>T. crassicaudatus</italic> and <italic>T. agri</italic> from China, Haiti, Indonesia, Iran, Niger and the USA, <italic>T. agri</italic> syn. n. is proposed as a junior synonym of <italic>T. crassicaudatus</italic>.</p>
</abstract>

Morphological and molecular analyses of plant-parasitic nematodes (PPN) from 12 sugarcane plantation sites of Tanganyika Planting Company (TPC) Limited in Kilimanjaro region of Tanzania revealed the presence of six PPN genera, i.e. Helicotylenchus, Hemicycliophora, Pratylenchus, Rotylenchulus, Scutellonema, and Tylenchorhynchus. The genera with the highest densities and present in virtually all samples were Pratylenchus and Rotylenchulus, and the most important species appeared to be R. parvus, P. zeae, T. crassicaudatus, and T. ventrosignatus. A total sequences of 11 partial ITS, 15 D2-D3 of 28S, and 6 partial 18S of rRNA gene, and 7 partial COI gene of mtDNA of these species were obtained in this study. Morphology and molecular data comparisons between the Tanzanian R. parvus and the South African R. parvus indicated that R. parvus is a cryptic species complex. Based on the results of morphological and molecular analyses of T. crassicaudatus and T. agri from China, Haiti, Indonesia, Iran, Niger and the USA, T. agri syn. n. is proposed as a junior synonym of T. crassicaudatus.

Plant-parasitic nematodes associated with sugarcane in Kilimanjaro, Tanzania

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

running-head

Plant-parasitic nematodes from sugarcane in Kilimanjaro:

Morphological and molecular analyses of plant-parasitic nematodes (PPN) from 12 sugarcane plantation sites of Tanganyika Planting Company (TPC) Limited in...

Plant-parasitic nematodes associated with sugarcane in Kilimanjaro, Tanzania

Published Online: Jul 06, 2020

Page range: 1 - 17

Received: Feb 13, 2020

DOI: https://doi.org/10.21307/jofnem-2020-059

Keywords
28S, ITS, TPC, Kilimanjaro, Phylogeny, Plant-parasitic nematodes, Sugarcane, Synonymy, Tanzania, Taxonomy

© 2020 Phougeishangbam Rolish Singh et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Plant-parasitic nematodes associated with sugarcane in Kilimanjaro, Tanzania

Published Online: Jul 06, 2020

Page range: 1 - 17

Received: Feb 13, 2020

DOI: https://doi.org/10.21307/jofnem-2020-059

Keywords28S, ITS, TPC, Kilimanjaro, Phylogeny, Plant-parasitic nematodes, Sugarcane, Synonymy, Tanzania, Taxonomy

© 2020 Phougeishangbam Rolish Singh et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
28S, ITS, TPC, Kilimanjaro, Phylogeny, Plant-parasitic nematodes, Sugarcane, Synonymy, Tanzania, Taxonomy