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Meloidogyne aegracyperi n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing yellow and purple nutsedge in New Mexico

J. D. Eisenback
J. D. Eisenback
, 
L. A. Holland
L. A. Holland
, 
J. Schroeder
J. Schroeder
, 
S. H. Thomas
S. H. Thomas
, 
J. M. Beacham
J. M. Beacham
, 
S. F. Hanson
S. F. Hanson
, 
V. S. Paes-Takahashi
V. S. Paes-Takahashi
 and 
P. Vieira
P. Vieira
   | Oct 25, 2019
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Article Category: Arts & Humanities
Published Online: Oct 25, 2019
Page range: 1 - 16
Received: Feb 01, 2019
DOI: https://doi.org/10.21307/jofnem-2019-071
© 2019 J. D. Eisenback et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Females of Meloidogyne aegracyperi n. sp.; (A-D) Light micrographs of whole specimens showing the typical shape of the body and the posterior protuberance containing the perineal pattern (scale bar=0.1 mm).
Females of Meloidogyne aegracyperi n. sp.; (A-D) Light micrographs of whole specimens showing the typical shape of the body and the posterior protuberance containing the perineal pattern (scale bar=0.1 mm).

Figure 2:

Scanning electron (SEM) and light micrographs of females of Meloidogyne aegracyperi n. sp.; (A) SEM of a whole female showing the location of the perineal pattern; (B) SEM of a perineal pattern situated on a protuberance of the posterior end of the body (magnification same as Fig. D); (C) SEM close-up of a perineal pattern; (D) Light micrograph showing the protuberance of the posterior end of the body containing the perineal pattern as seen in Fig. B.
Scanning electron (SEM) and light micrographs of females of Meloidogyne aegracyperi n. sp.; (A) SEM of a whole female showing the location of the perineal pattern; (B) SEM of a perineal pattern situated on a protuberance of the posterior end of the body (magnification same as Fig. D); (C) SEM close-up of a perineal pattern; (D) Light micrograph showing the protuberance of the posterior end of the body containing the perineal pattern as seen in Fig. B.

Figure 3:

Light micrographs of perineal patterns of females of Meloidogyne aegracyperi n. sp.
Light micrographs of perineal patterns of females of Meloidogyne aegracyperi n. sp.

Figure 4:

Light (LM) and scanning electron micrographs (SEM) of females of Meloidogyne aegracyperi n. sp.; (A) LM of posterior protuberance containing the perineal pattern with the anus and vulva marked by arrows; (B) SEM of posterior protuberance showing the swollen vulval lips and tail terminus; (C) SEM of the extracted cuticular lining of the esophagus with the stylet and showing the triradiate plates of the metacorpus and the vesicles in the lumen lining that are usually contained in the anterior region of the metacorpus; (D) SEM of an extracted stylet showing the angular edges on the knobs and the long dorsal esophageal gland orifice; (E) LM of the anterior end of the female showing the stylet and metacorpus.
Light (LM) and scanning electron micrographs (SEM) of females of Meloidogyne aegracyperi n. sp.; (A) LM of posterior protuberance containing the perineal pattern with the anus and vulva marked by arrows; (B) SEM of posterior protuberance showing the swollen vulval lips and tail terminus; (C) SEM of the extracted cuticular lining of the esophagus with the stylet and showing the triradiate plates of the metacorpus and the vesicles in the lumen lining that are usually contained in the anterior region of the metacorpus; (D) SEM of an extracted stylet showing the angular edges on the knobs and the long dorsal esophageal gland orifice; (E) LM of the anterior end of the female showing the stylet and metacorpus.

Figure 5:

Light micrographs of a whole male specimen with an enlarged view of the anterior end of Meloidogyne aegracyperi n. sp.
Light micrographs of a whole male specimen with an enlarged view of the anterior end of Meloidogyne aegracyperi n. sp.

Figure 6:

(A) Scanning electron micrograph of the anterior end of a second-stage juvenile of Meloidogne aegracyperi n. sp. (B) Light micrographs (LM) of eggs of M. aegracyperi n. sp. showing the variation in size for three eggs in the two-cell stage. Light micrographs of second-stage juveniles of M. aegracyperi n. sp.
(A) Scanning electron micrograph of the anterior end of a second-stage juvenile of Meloidogne aegracyperi n. sp. (B) Light micrographs (LM) of eggs of M. aegracyperi n. sp. showing the variation in size for three eggs in the two-cell stage. Light micrographs of second-stage juveniles of M. aegracyperi n. sp.

Figure 7:

Light micrographs of the anterior ends and tails of second-stage juveniles of Meloidogyne aegracyperi n. sp.
Light micrographs of the anterior ends and tails of second-stage juveniles of Meloidogyne aegracyperi n. sp.

Figure 8:

Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including NSRKN (Meloidogyne aegracyperi n. sp.) and comparison 18S rRNA sequences. The tree with the highest log likelihood (−2,286.9884) is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.
Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including NSRKN (Meloidogyne aegracyperi n. sp.) and comparison 18S rRNA sequences. The tree with the highest log likelihood (−2,286.9884) is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.

Figure 9:

Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including NSRKN (Meloidogyne aegracyperi n. sp.) and comparison Hsp90 sequences. The tree with the highest log likelihood (−2,926.7701) is shown. The bootstrap support values (500 bootstraps) are shown next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.
Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including NSRKN (Meloidogyne aegracyperi n. sp.) and comparison Hsp90 sequences. The tree with the highest log likelihood (−2,926.7701) is shown. The bootstrap support values (500 bootstraps) are shown next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.

Figure 10:

Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including Meloidogyne aegracyperi n. sp. and comparison of the alignment of the D2-D3 (28S rRNA) sequences. The tree with the highest log likelihood is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.
Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including Meloidogyne aegracyperi n. sp. and comparison of the alignment of the D2-D3 (28S rRNA) sequences. The tree with the highest log likelihood is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.

Figure 11:

Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including Meloidogyne aegracyperi n. sp. and comparison of the alignment of the ITS sequences. The tree with the highest log likelihood is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.
Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including Meloidogyne aegracyperi n. sp. and comparison of the alignment of the ITS sequences. The tree with the highest log likelihood is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.

Figure 12:

Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including Meloidogyne aegracyperi n. sp. and comparison of the alignment of the COII-16S rRNA sequences. The tree with the highest log likelihood is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.
Maximum likelihood phylogenetic analysis of evolutionary relationships of closely related Meloidogyne species including Meloidogyne aegracyperi n. sp. and comparison of the alignment of the COII-16S rRNA sequences. The tree with the highest log likelihood is shown. The bootstrap support values (500 bootstraps) are shown at nodes next to the branches. Branch lengths are proportional to the distances. Genbank accession numbers follow each species.
eISSN:
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Language:
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Journal Subjects:
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