<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Entomopathogenic nematodes have been evaluated for control of mosquito species for decades. Depending on the nematode and mosquito involved, mortality rates of larvae (L) may reach 100% in vitro. Nonetheless, nematode efficacy at oviposition sites has rarely been assessed. <italic>Heterorhabditis indica</italic> LPP35 has been shown to kill over 75% of <italic>Aedes aegypti</italic> L3/L4 in cups and bottles outdoors. To assess its efficacy in indoor oviposition sites, different types/sizes of floor drains and pot saucers, and 65 liter water barrels, were infested with L3/L4 and treated with two doses of infective juveniles (IJs). In floor drains, mortality rates varied from 45 to 82%, with better results in the smallest drains. The adjustable dose of 25 IJs/cm<sup>2</sup> of the drain’s bottom internal surface gave better results than the fixed dose of 100 IJs/larva. Mortality rates were only 28 to 53% and 0.1 to 1.7% in pot saucers and water barrels, respectively, probably because ridges and grooves that marked the bottom internal surface of these containers hindered the encounter of larvae and IJs.</p>
</abstract>

Entomopathogenic nematodes have been evaluated for control of mosquito species for decades. Depending on the nematode and mosquito involved, mortality rates of larvae (L) may reach 100% in vitro. Nonetheless, nematode efficacy at oviposition sites has rarely been assessed. Heterorhabditis indica LPP35 has been shown to kill over 75% of Aedes aegypti L3/L4 in cups and bottles outdoors. To assess its efficacy in indoor oviposition sites, different types/sizes of floor drains and pot saucers, and 65 liter water barrels, were infested with L3/L4 and treated with two doses of infective juveniles (IJs). In floor drains, mortality rates varied from 45 to 82%, with better results in the smallest drains. The adjustable dose of 25 IJs/cm2 of the drain’s bottom internal surface gave better results than the fixed dose of 100 IJs/larva. Mortality rates were only 28 to 53% and 0.1 to 1.7% in pot saucers and water barrels, respectively, probably because ridges and grooves that marked the bottom internal surface of these containers hindered the encounter of larvae and IJs.

Efficacy of <italic>Heterorhabdits indica</italic> LPP35 against <italic>Aedes aegypti</italic> in domiciliary oviposition sites

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Efficacy of  LPP35 against  in domiciliary oviposition sites

running-head

Entomopathogenic nematodes have been evaluated for control of mosquito species for decades. Depending on the nematode and mosquito involved, mortality rates of...

Sizes (bottom internal surface area, in cm²)
Treatments	Small (13.3)	Medium (87.9)	Large (245)
100 IJs/larva
Assay 1
Treated	46a	29a	35a
Control	3b	11b	14b
Assay 2
Treated	53a	53a	35a
Control	18b	13b	8b
25 IJs/cm ²
Assay 1
Treated	31a	44a	38a
Control	0b	0b	0b
Assay 2
Treated	28a	37a	30a
Control	0b	0b	0b

Efficacy of Heterorhabdits indica LPP35 against Aedes aegypti in domiciliary oviposition sites

Article Category: Arts & Humanities

Published Online: Jul 23, 2019

Page range: 1 - 7

Received: May 01, 2019

DOI: https://doi.org/10.21307/jofnem-2019-050

© 2019 Bruna Silva et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Mortality rate (%) of Aedes aegypti L3/L4 in pot saucers, seven days after treatment with Heterorhabditis indica LPP35 infective juveniles (IJs), at two distinct doses.