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Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

Farhana Begum
Farhana Begum
, 
John Fosu-Nyarko
John Fosu-Nyarko
, 
Shashi Sharma
Shashi Sharma
, 
Bill Macleod
Bill Macleod
, 
Sarah Collins
Sarah Collins
 and 
Michael G. K. Jones
Michael G. K. Jones
   | Jul 29, 2019
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Article Category: Arts & Humanities
Published Online: Jul 29, 2019
Page range: 1 - 15
Received: Feb 17, 2019
DOI: https://doi.org/10.21307/jofnem-2019-046
© 2019 Farhana Begum et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Locations of the four wheat/barley paddocks in the Western Australian grainbelt where P. curvicauda samples were isolated. The four sites are circled; Pingelly, Williams, Arthur River, and Katanning (https://maps-australia.com/south-western-australia-map).
Locations of the four wheat/barley paddocks in the Western Australian grainbelt where P. curvicauda samples were isolated. The four sites are circled; Pingelly, Williams, Arthur River, and Katanning (https://maps-australia.com/south-western-australia-map).

Figure 2:

Light micrograph and line drawings of P. curvicauda from Pingelly, Western Australia [I] (A). Whole female adult nematode, (B) head region, (C) tail region (scale bar = 100 μm). [II]. Hand drawings of P. curvicauda from Pingelly, Western Australia: (A) head region, (B) Esophageal region, (C) vulva region, (D and E) tail region (Illustrations by the late Dr M. R. Siddiqi).
Light micrograph and line drawings of P. curvicauda from Pingelly, Western Australia [I] (A). Whole female adult nematode, (B) head region, (C) tail region (scale bar = 100 μm). [II]. Hand drawings of P. curvicauda from Pingelly, Western Australia: (A) head region, (B) Esophageal region, (C) vulva region, (D and E) tail region (Illustrations by the late Dr M. R. Siddiqi).

Figure 3:

Scanning electron micrographs of an adult female P. curvicauda from Pingelly, Western Australia. (A) Whole nematode body, (B-C) En face view, (D) lateral field of the vulval region, (E) tail terminus. (F) tail region, (G) vulval region; (H) lateral field at middle of the body.
Scanning electron micrographs of an adult female P. curvicauda from Pingelly, Western Australia. (A) Whole nematode body, (B-C) En face view, (D) lateral field of the vulval region, (E) tail terminus. (F) tail region, (G) vulval region; (H) lateral field at middle of the body.

Figure 4:

Light micrographs of the tail shapes of P. curvicauda and P. thornei (Western Australia) compared to nematode representative of P. neglectus and P. penetrans (A–C) P. curvicauda from Pingelly – (A) entire female body, (B) head region and (C) tail region (D–F) P. neglectus, (D) entire female body, (E) head region and (F) tail region (G–I) P. penetrans, (G) entire female body, (H) head region and (I) tail region (J–L) P. thornei (J) entire female body, (K) head region and (L) tail region (scale bar: A–C = 50 µm and D–L = 100 µm).
Light micrographs of the tail shapes of P. curvicauda and P. thornei (Western Australia) compared to nematode representative of P. neglectus and P. penetrans (A–C) P. curvicauda from Pingelly – (A) entire female body, (B) head region and (C) tail region (D–F) P. neglectus, (D) entire female body, (E) head region and (F) tail region (G–I) P. penetrans, (G) entire female body, (H) head region and (I) tail region (J–L) P. thornei (J) entire female body, (K) head region and (L) tail region (scale bar: A–C = 50 µm and D–L = 100 µm).

Figure 5:

Molecular phylogenetic trees of representative sequences of the partial 18S-ITS1-5.8S-ITS2-partial 28S region of the rDNA of P. curvicauda isolates from soils of the grainbelt collected from four known (Arthur River, Pingelly, Williams, Katanning) and one unknown (UN) location in Western Australia. (A) Phylogenetic tree of sequences of the partial 18S-ITS1-5.8S-ITS2-partial regions, (B) Phylogenetic tree of sequences of ITS1 region, (C) Phylogenetic tree of sequences of the ITS2 region.
Molecular phylogenetic trees of representative sequences of the partial 18S-ITS1-5.8S-ITS2-partial 28S region of the rDNA of P. curvicauda isolates from soils of the grainbelt collected from four known (Arthur River, Pingelly, Williams, Katanning) and one unknown (UN) location in Western Australia. (A) Phylogenetic tree of sequences of the partial 18S-ITS1-5.8S-ITS2-partial regions, (B) Phylogenetic tree of sequences of ITS1 region, (C) Phylogenetic tree of sequences of the ITS2 region.

Figure 6:

Molecular phylogenetic tree of P. curvicauda isolates and sequences representative of 35 Pratylenchus species using the partial 18S-ITS1-5.8S-ITS2-28S region of the rDNA. Bootstrap values 30 and above are shown.
Molecular phylogenetic tree of P. curvicauda isolates and sequences representative of 35 Pratylenchus species using the partial 18S-ITS1-5.8S-ITS2-28S region of the rDNA. Bootstrap values 30 and above are shown.

Figure 7:

Molecular phylogenetic tree of P. curvicauda isolates and sequences representative of 40 Pratylenchus species using the 28S-D3 region of the rDNA. Boostrap values 30 and above are shown.
Molecular phylogenetic tree of P. curvicauda isolates and sequences representative of 40 Pratylenchus species using the 28S-D3 region of the rDNA. Boostrap values 30 and above are shown.
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Language:
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