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Molecular and morphological characterization of Paurodontella composticola n. sp. (Nematoda: Hexatylina, Sphaerulariidae) from Iran

Mehrab Esmaeili
Mehrab Esmaeili
, 
Ramin Heydari
Ramin Heydari
, 
Ahmad Kheiri
Ahmad Kheiri
 and 
Weimin Ye
Weimin Ye
   | Jun 06, 2019
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Article Category: Arts & Humanities
Published Online: Jun 06, 2019
Page range: 1 - 12
Received: Nov 20, 2018
DOI: https://doi.org/10.21307/jofnem-2019-034
© 2019 Mehrab Esmaeili et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Line drawing of Paurodontella composticola n. sp. (A): female entire body; (B): male entire body; (C): stylet; (D): medium bulb region of female; (E): female esophageal region; (F): male posterior body; (G): female posterior region; (H): lateral field of female. (All scale bars 30 μ m, except for C = 10 μ m; D = 20 μ m).
Line drawing of Paurodontella composticola n. sp. (A): female entire body; (B): male entire body; (C): stylet; (D): medium bulb region of female; (E): female esophageal region; (F): male posterior body; (G): female posterior region; (H): lateral field of female. (All scale bars 30 μ m, except for C = 10 μ m; D = 20 μ m).

Figure 2:

Photomicrographs of Paurodontella composticola n. sp. (A): female anterior end; (B, C): basal bulb region of female; (D): vulval region; (E): lateral field of female; (F): vulva to anus distance; (G): female posterior region; (H, I): male posterior body. (All scale bars 20 μ m).
Photomicrographs of Paurodontella composticola n. sp. (A): female anterior end; (B, C): basal bulb region of female; (D): vulval region; (E): lateral field of female; (F): vulva to anus distance; (G): female posterior region; (H, I): male posterior body. (All scale bars 20 μ m).

Figure 3:

Bayesian consensus tree inferred from 28S D2/D3 rDNA under TVM + I + G model (−lnL = 13,024.7812; AIC = 26,067.5625; freqA = 0.1584; freqC = 0.2128; freqG = 0.3327; freqT = 0.2961; R(a) = 1.0917; R(b) = 5.2109; R(c) = 2.5802; R(d) = 0.8631; R(e) = 5.2109; R(f) = 1; Pinva = 0.1293; Shape = 0.8648). Posterior probability values exceeding 50% are given on appropriate clades. The new sequence is in boldface.
Bayesian consensus tree inferred from 28S D2/D3 rDNA under TVM + I + G model (−lnL = 13,024.7812; AIC = 26,067.5625; freqA = 0.1584; freqC = 0.2128; freqG = 0.3327; freqT = 0.2961; R(a) = 1.0917; R(b) = 5.2109; R(c) = 2.5802; R(d) = 0.8631; R(e) = 5.2109; R(f) = 1; Pinva = 0.1293; Shape = 0.8648). Posterior probability values exceeding 50% are given on appropriate clades. The new sequence is in boldface.

Species used for the analysis of phylogenetic relationships and the accession numbers for LSU sequences deposited in GenBank Species.

Accession number Authors Species Accession number Authors
Abursanema iranicum KF885742 Yaghoubi et al. (2014) Howardulla sp. JX291131 Chizhov et al. (2012)
Anguina sp. KU317615 unpublished data Litylenchus coprosma GU727547 Zhao et al. (2012)
Anguina tritici DQ328723, KC818620 Subbotin et al. (2006) Nothotylenchus persicus KT149799 Esmaeili et al. (2015)
Anguinata KY067443 unpublished data Nothotylenchus phoneixae KX549319 Esmaeili et al. (2017c)
Cephalenchus sp. KU723245 Pereira and Baldwin (2016) Nothotylenchus sp. MH243749 unpublished data
Cephalenchus cephalodiscus KX685166 Pereira et al. (2017) Paurodontella composticolla n. sp. MH427864 Present study
Cephalenchus daisuce KX462033 Pereira et al. (2017) Paurodontella gilanica MF543010 Yaghoubi et al. (2018)
Cephalenchus nemoralis KU723245 Pereira and Baldwin (2016) Paurodontella iranica KP642168 Golhasan et al. (2016)
Deladenus siricidicola AY633444 Ye et al. (2007) Paurodontella parapitica KU522237 Esmaeili et al. (2016b)
Deladenus sp. JX104332 unpublished data Paurodontella persica KP000034 Esmaeili et al. (2017a)
Deladenus sp. JX104322 Morris et al. (2013) Paurodontoides sp. MG836264 Esmaeili et al. (2018)
Ditylenchus arachis KX426052, KX426054 Wang et al. (2017) Rubzovinema sp. KF155283 Koshel et al. (2014)
Ditylenchus destructor EU400626 Wang et al. (2017) Rubzovinema sp. KF373736 Koshel et al. (2014)
Ditylenchus dipsaci FJ707363, KT806479, JF327763, JF327765, Douda et al. (2013) Sphaerularia cf. bombi AB733664, AB733665, DQ328726 Ye et al. (2007)
Ditylenchus gigas HQ219217, KC310734 Vovlas et al. (2011) Sphaerularia vespae AB300596 Ye et al. (2007)
Ditylenchus oncogenus KF612015 Vovlas et al. (2016) Sphaerularioidea KR920361 unpublished data
Ditylenchus persicus KX463285 Esmaeili et al. (2016a) Subanguina moxae JN885540 Yao et al. (2012)
Ditylenchus stenurus KX400577 Esmaeili et al. (2017b) Subanguina radicicola JN885539 Yao et al. (2012)
Ditylenchus weischeri MG551886 Madani and Tenuta (2017) Subanguina sp. KT205566, KT205560, KX776479 unpublished data
Fergusobia sp. AY633446 Ye et al. (2007) Travassosinema claudiae KX844645 Morffe and Hasegawa (2017)
Tylenchida sp. FJ661086 unpublished data Travassosinema sp. LC214839 unpublished data
Tylenchomorpha sp. LC147027 unpublished data – – –

Morphometrics of Paurodontella composticola n. sp.

Female Male
Character Holotype Paratypes Paratypes
n – 10 2
L 1,005 940 ± 95.9 (803–1,053) 620–793
a 24.5 25.9 ± 4.2 (18.6–31.5) 23.0–30.1
b 6.7 5.1 ± 0.6 (5.4–6.9) 4.2–5.1
c 22.3 24.5 ± 4.4 (17.8–32.1) 20.7–22.5
c' 2.0 2.1 ± 0.4 (1.5–2.5) 1.8–2.4
V or T 89.5 90.0 ± 1.2 (78.4–91.2) 40.6–50.0
Lip region height 3 2.9 ± 0.2 (2.5–3.0) 3.0
Lip region width 8.5 6.6 ± 0.1 (6.0–7.0) 7.0–8.0
Stylet length 9 8.8 ± 0.5 (8.0–9.0) 8.0–8.5
Nerve ring from anterior end 85 91.3 ± 12.0 (79–103) 85–93
E. pore from anterior end 136 129.6 ± 10.2 (116–144) 92–124
Pharynx length 150 154.9 ± 16.7 (136–182) 140–156
PUS length 5 5.6 ± 1.2 (5.0–8.0) –
Ovary length or testis 624 585 ± 80.8 (408–656) 300–310
Body diameter at vulva (VBD) 33 29.4 ± 5.7 (23–40) –
Vulva to anus 57 52.8 ± 3.8 (46–57) –
Anal (cloacal) body diam. 22 19 ± 2.8 (16–24) 14–17
Tail length 45 39.3 ± 6.0 (26–45) 30–33
Spicules length (arc line) – – 24–25
Gubernaculum length – – 7.0–8.0
Bursa (% of tail) – – 96
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Language:
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