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Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

Puneet Kumar
Puneet Kumar
, 
Wajih Jamal
Wajih Jamal
, 
Vishal S. Somvanshi
Vishal S. Somvanshi
, 
Khushbu Chauhan
Khushbu Chauhan
 and 
Sabia Mumtaz
Sabia Mumtaz
   | Apr 02, 2019
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Article Category: Arts & Humanities
Published Online: Apr 02, 2019
Page range: 1 - 11
Received: Sep 03, 2018
DOI: https://doi.org/10.21307/jofnem-2019-004
© 2019 Puneet Kumar et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Oscheius indicus n. sp. (A) Entire male; (B) Entire female; (C) Pharyngeal region; (D) Anterior region; (E) Female reproductive system; (F) Male posterior region showing spicules and gubernaculum; (G) Male tail in dorso-ventral view (genital papillae and bursa); (H) Female posterior region.
Oscheius indicus n. sp. (A) Entire male; (B) Entire female; (C) Pharyngeal region; (D) Anterior region; (E) Female reproductive system; (F) Male posterior region showing spicules and gubernaculum; (G) Male tail in dorso-ventral view (genital papillae and bursa); (H) Female posterior region.

Figure 2

Oscheius indicus n. sp. (A, B) Anterior region showing stoma; (C) Posterior pharynx.; (D) Genital papillae; (E) Anterior pharynx; (F) Vulva; (G) Male posterior region; (H) Female posterior region; (I) Lateral lines. (Scale bar: 20 μm).
Oscheius indicus n. sp. (A, B) Anterior region showing stoma; (C) Posterior pharynx.; (D) Genital papillae; (E) Anterior pharynx; (F) Vulva; (G) Male posterior region; (H) Female posterior region; (I) Lateral lines. (Scale bar: 20 μm).

Figure 3

Oscheius indicus n. sp. (A) Enface view showing six labial papillae (a to f), (B) Crochet needle-shaped spicules (C) Male posterior region showing genital papillae (GP1 to GP9), (D) Female posterior region showing anal opening (Scale bar: A = 2 μm; B & C = 10 μm, D = 5 μm).
Oscheius indicus n. sp. (A) Enface view showing six labial papillae (a to f), (B) Crochet needle-shaped spicules (C) Male posterior region showing genital papillae (GP1 to GP9), (D) Female posterior region showing anal opening (Scale bar: A = 2 μm; B & C = 10 μm, D = 5 μm).

Figure 4

Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using 28S D2/D3 extension region of 28 rDNA gene. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.
Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using 28S D2/D3 extension region of 28 rDNA gene. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.

Figure 5

Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using ITS region. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.
Molecular phylogenetic relationship of Oscheius indicus n. sp. (highlighted in bold) inferred using ITS region. The evolutionary history was inferred by the Bayesian analysis using the General Time Reversible substitution model with gamma-distributed rate variation across sites and a proportion of invariable sites (GTR+G+I). The posterior probability values exceeding 50% are indicated at appropriate clades. The scale bar indicates expected changes per site. Orange and green shaded boxes indicate insectivora and dolichura group, respectively.

Morphometric data of Oscheius indicus sp. n. Measurements are in µm and in the form: mean ± standard deviation (range).

Characters Holotype female Paratype females Paratype males
n 1 14 14
L 1,320 1,272.3 ± 111.7 (1,072–1,480) 1,062.7 ± 104.5 (886–1,209)
a 20.3 18.9 ± 1.1 (16.8–20.6) 19.6 ± 1.6 (17.4–22.4)
b 6.5 6.5 ± 0.4 (5.7–7.1) 6.2 ± 0.6 (4.9–6.9)
c 9.9 8.9 ± 0.9 (7.5–10.4) 31.2 ± 2.8 (26.1–36.2)
c’ 5.3 5.8 ± 0.4 (5.0–6.6) 1.3 ± 0.1 (1.2–1.4)
V 50.2 48.2 ± 1.8 (45.4–50.5) –
Maximum body diam. 65 67.8 ± 20.2 (59–82) 54.4 ± 16.3 (46–64)
Lip width 12 12.6 ± 1.1 (11–15) 11.6 ± 0.7 (11–13)
Length of stoma 16 15.4 ± 1.7 (13–18) 15.6 ± 0.9 (14–17)
Corpus 123 117.7 ± 6.1 (108–127) 105.4 ± 4.0 (101–114)
Isthmus 42 40.7 ± 3.4 (37–47) 35.4 ± 3.5 (31–41)
Basal bulb length 37 36.4 ± 2.2 (33–41) 29.9 ± 1.4 (27–32)
Basal bulb width 29 27.6 ± 1.8 (24–30) 23.8 ± 0.9 (22–25)
Pharynx 202 194.8 ± 9.8 (181–210) 172.4 ± 6.0 (159–181)
Excretory pore from anterior end 201 179.9 ± 15.1 (154–201) 173.6 ± 9.7 (158–187)
Nerve ring from anterior end 162 142.0 ± 14.1 (118–162) 136.7 ± 7.6 (122–146)
Anterior gonad 524 558.2 ± 43.2 (501–640) –
Posterior gonad 352 504.4 ± 90.3 (324–614) –
Vulva body diam. 64 66.4 ± 5.5 (58–80) –
Vulva-anus distance 525 512.1 ± 40.2 (447–576) –
Rectum 72 67.9 ± 5.8 (58–76) 54.3 ± 4.3 (46–61)
Tail length 133 142.6 ± 8.9 (131–156) 34.2 ± 2.3 (32–39)
Anal body diameter 25 24.8 ± 1.4 (23–28) 25.6 ± 1.2 (24–28)
Pharynx gonad distance 175 118.0 ± 31.6 (89–175) 82.8 ± 17.2 (61–116)
Testis _ – 883.0 ± 82.1 (760–1,035)
Spicule length along axis _ – 60.0 ± 4.2 (54–66)
Gubernaculum _ – 25.1 ± 1.2 (24–28)
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