<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>
<italic>Citrullus amarus</italic> (<italic>CA</italic>) (previously known as <italic>Citrullus lanatus</italic> var. <italic>citroides</italic>) accessions collected in southern Africa are known to have resistance to root-knot nematodes (RKN) and are suitable rootstocks for grafted watermelon. The objective of this study was to conduct a comparative metabolomics analysis and identify unique metabolites in roots of <italic>CA</italic> accessions versus roots of watermelon cultivars (<italic>Citrullus lanatus</italic> (Thunb.) Matsum. and Nakai var. <italic>lanatus; CL</italic>). Nuclear magnetic resonance (NMR) technology and principal component analysis (PCA) were used to analyze and compare metabolic profiles of seven <italic>CA</italic> accessions resistant to RKN along with two RKN-susceptible watermelon cultivars (Charleston Gray and Crimson Sweet). Calculation of the Mahalanobis distance revealed that the <italic>CA</italic> United States Plant Introduction (PI) 189225 (Line number 1832) and PI 482324 (1849) have the most distinct metabolic profiles compared with the watermelon cultivars Charleston Gray and Crimson Sweet, respectively. Several amino acids identified in the <italic>CA</italic> accessions were reported in previous studies to have a nematicidal effect. The results in this study indicate that roots of watermelon accessions collected in the wild are rich in metabolic compounds. These metabolic compounds may have been diminished in watermelon cultivars as a consequence of many years of cultivation and selection for desirable fruit qualities.</p>
</abstract>

Citrullus amarus (CA) (previously known as Citrullus lanatus var. citroides) accessions collected in southern Africa are known to have resistance to root-knot nematodes (RKN) and are suitable rootstocks for grafted watermelon. The objective of this study was to conduct a comparative metabolomics analysis and identify unique metabolites in roots of CA accessions versus roots of watermelon cultivars (Citrullus lanatus (Thunb.) Matsum. and Nakai var. lanatus; CL). Nuclear magnetic resonance (NMR) technology and principal component analysis (PCA) were used to analyze and compare metabolic profiles of seven CA accessions resistant to RKN along with two RKN-susceptible watermelon cultivars (Charleston Gray and Crimson Sweet). Calculation of the Mahalanobis distance revealed that the CA United States Plant Introduction (PI) 189225 (Line number 1832) and PI 482324 (1849) have the most distinct metabolic profiles compared with the watermelon cultivars Charleston Gray and Crimson Sweet, respectively. Several amino acids identified in the CA accessions were reported in previous studies to have a nematicidal effect. The results in this study indicate that roots of watermelon accessions collected in the wild are rich in metabolic compounds. These metabolic compounds may have been diminished in watermelon cultivars as a consequence of many years of cultivation and selection for desirable fruit qualities.

NMR Analysis Reveals a Wealth of Metabolites in Root-Knot Nematode Resistant Roots of <italic>Citrullus amarus</italic> Watermelon Plants

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

NMR Analysis Reveals a Wealth of Metabolites in Root-Knot Nematode Resistant Roots of  Watermelon Plants

running-head

Citrullus amarus (CA) (previously known as Citrullus lanatus var. citroides) accessions collected in southern Africa are known to have resistance to root-knot...

NMR Analysis Reveals a Wealth of Metabolites in Root-Knot Nematode Resistant Roots of Citrullus amarus Watermelon Plants

Published Online: Oct 17, 2018

Page range: 303 - 316

DOI: https://doi.org/10.21307/jofnem-2018-030

Keywords
Biochemical pathway analysis, Nuclear magnetic resonance, Root-knot nematodes, Watermelon roots

© 2018 Mihail Kantor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

NMR Analysis Reveals a Wealth of Metabolites in Root-Knot Nematode Resistant Roots of Citrullus amarus Watermelon Plants

Published Online: Oct 17, 2018

Page range: 303 - 316

DOI: https://doi.org/10.21307/jofnem-2018-030

KeywordsBiochemical pathway analysis, Nuclear magnetic resonance, Root-knot nematodes, Watermelon roots

© 2018 Mihail Kantor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Biochemical pathway analysis, Nuclear magnetic resonance, Root-knot nematodes, Watermelon roots