<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In a search for an entomopathogenic nematode to control cranberry insect pests, three <italic>Oscheius</italic> populations (Rhabditidae) were recovered through the <italic>Galleria-</italic>bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and mitochondrial cytochrome oxidase subunit 1 (CoxI) genes revealed this as <italic>Oscheius onirici,</italic> a species recently described from a karst cave soil of central Italy. The species belongs to the <italic>dolichura-</italic>group and is characterized by its DNA sequences; hermaphroditic reproduction; and males not found. A <italic>Bacillus</italic>-like bacterium appears to be associated with this nematode based on our microscopic and SEM observations; however its identity and persistent association with the nematode has not been confirmed. Nonetheless, this nematode is capable of infecting and killing the sparganothis fruitworm <italic>Sparganothis sulfureana</italic> Clemens (Lepidoptera: Tortricidae), the brown-banded cockroach <italic>Supella longipalpa</italic> Fabricius (Blattodea: Ectobiidae), and the cranberry fruitworm <italic>Acrobasis vaccinii</italic> Riley (Lepidoptera: Pyralidae), under laboratory conditions, and each in less than 72 hr. The mealworm <italic>Tenebrio molitor</italic> Linnaeus (Coleoptera: Tenebrionidae) and the greater wax moth <italic>Galleria mellonella</italic> Linnaeus (Lepidoptera: Pyralidae), are also susceptible, but take 3.5 and 5.2 days to die, respectively. This species is a new potential bio-control agent on insects.</p>
</abstract>

In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the Galleria-bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and mitochondrial cytochrome oxidase subunit 1 (CoxI) genes revealed this as Oscheius onirici, a species recently described from a karst cave soil of central Italy. The species belongs to the dolichura-group and is characterized by its DNA sequences; hermaphroditic reproduction; and males not found. A Bacillus-like bacterium appears to be associated with this nematode based on our microscopic and SEM observations; however its identity and persistent association with the nematode has not been confirmed. Nonetheless, this nematode is capable of infecting and killing the sparganothis fruitworm Sparganothis sulfureana Clemens (Lepidoptera: Tortricidae), the brown-banded cockroach Supella longipalpa Fabricius (Blattodea: Ectobiidae), and the cranberry fruitworm Acrobasis vaccinii Riley (Lepidoptera: Pyralidae), under laboratory conditions, and each in less than 72 hr. The mealworm Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) and the greater wax moth Galleria mellonella Linnaeus (Lepidoptera: Pyralidae), are also susceptible, but take 3.5 and 5.2 days to die, respectively. This species is a new potential bio-control agent on insects.

Incidence of <italic>Oscheius onirici</italic> (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Incidence of  (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

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In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the...

Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Article Category: research-article

Published Online: May 31, 2018

Page range: 9 - 26

Accepted: Nov 19, 2017

DOI: https://doi.org/10.21307/jofnem-2018-004

Keywords
Cranberry, characterization, DNA sequencing, entomopathogenic nematode, Dolichura-group, molecular phylogeny, morphology, rDNA

© 2018 Weimin Ye et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Incidence of Oscheius onirici (Nematoda: Rhabditidae), a potentially entomopathogenic nematode from the marshlands of Wisconsin, USA

Article Category: research-article

Published Online: May 31, 2018

Page range: 9 - 26

Accepted: Nov 19, 2017

DOI: https://doi.org/10.21307/jofnem-2018-004

KeywordsCranberry, characterization, DNA sequencing, entomopathogenic nematode, Dolichura-group, molecular phylogeny, morphology, rDNA

© 2018 Weimin Ye et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Cranberry, characterization, DNA sequencing, entomopathogenic nematode, Dolichura-group, molecular phylogeny, morphology, rDNA