<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Fourteen populations of <italic>Meloidogyne graminicola</italic> were collected from different agroecological regions of India. Morphological and morphometrical comparisons were made for various nematode life stages. Three populations (Hisar, New Delhi, and Samastipur) were different from typical <italic>M. graminicola</italic> on the basis of the length of eggs; J2 length, <italic>a</italic>-value, hyaline tail portion; male length, distance up to excretory pore, spicule and gubernaculum lengths; female length and width, stylet length, distance up to excretory pore, EPST (distance of excretory pore from anterior end / stylet length [females]) ratio, and vulval length. Morphological and morphometrical comparison with closely related species <italic>M. graminis</italic>, <italic>M. oryzae</italic>, <italic>M. salasi</italic>, <italic>M. triticoryzae</italic>, and <italic>M. lini</italic> clustered these populations into two groups: Anand, Bhubaneswar, Hyderabad, Jammu, Jorhat, Kalyani, Kanpur, Ludhiana, Mandya, Palampur, Vellayani grouped with <italic>M. graminicola</italic>, <italic>M. triticoryzae</italic> and <italic>M. salasi</italic>; whereas, Hisar, New Delhi, Samastipur grouped with <italic>M. oryzae</italic> and
<italic>M. graminis</italic>. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to <italic>M. graminicola</italic>.</p>
</abstract>

Fourteen populations of Meloidogyne graminicola were collected from different agroecological regions of India. Morphological and morphometrical comparisons were made for various nematode life stages. Three populations (Hisar, New Delhi, and Samastipur) were different from typical M. graminicola on the basis of the length of eggs; J2 length, a-value, hyaline tail portion; male length, distance up to excretory pore, spicule and gubernaculum lengths; female length and width, stylet length, distance up to excretory pore, EPST (distance of excretory pore from anterior end / stylet length [females]) ratio, and vulval length. Morphological and morphometrical comparison with closely related species M. graminis, M. oryzae, M. salasi, M. triticoryzae, and M. lini clustered these populations into two groups: Anand, Bhubaneswar, Hyderabad, Jammu, Jorhat, Kalyani, Kanpur, Ludhiana, Mandya, Palampur, Vellayani grouped with M. graminicola, M. triticoryzae and M. salasi; whereas, Hisar, New Delhi, Samastipur grouped with M. oryzae and
M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of <italic>Meloidogyne graminicola</italic>

Division of Plant Breeding & Genetics, Faculty of Agriculture, Sher-e-Kashmir University of Agriculture & Technology

ICAR - Indian Agricultural Research Institute, Division of Nematology

ICAR - Indian Agricultural Research Institute

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of 

Fourteen populations of Meloidogyne graminicola were collected from different agroecological regions of India. Morphological and morphometrical comparisons...

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

Published Online: Dec 03, 2017

Page range: 168 - 176

Received: Mar 26, 2017

DOI: https://doi.org/10.21307/jofnem-2017-071

Keywords
agglomerative hierarchical clustering, diversity, internal transcribed spacer, molecular characterization, morphology, morphometrics, multivariate analysis, phylogenetics, variation

© 2017 Rajan Salalia et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

Published Online: Dec 03, 2017

Page range: 168 - 176

Received: Mar 26, 2017

DOI: https://doi.org/10.21307/jofnem-2017-071

Keywordsagglomerative hierarchical clustering, diversity, internal transcribed spacer, molecular characterization, morphology, morphometrics, multivariate analysis, phylogenetics, variation

© 2017 Rajan Salalia et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
agglomerative hierarchical clustering, diversity, internal transcribed spacer, molecular characterization, morphology, morphometrics, multivariate analysis, phylogenetics, variation