<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Gracilacus wuae</italic> n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet ranging from 80 to 93 μm long, the lip region not offset from the body contour, without lateral lips but with large and flat submedian lobes, the mouth opening slit-like elongated laterally and surrounded by lateral flaps, the excretory pore is anterior to the knobs of the stylet; males without stylet and the pharynx degenerated. The fourth-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of the genital primordia. The third-stage juveniles are similar to females but smaller. Phylogenetic studies using the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer (ITS) sequences collectively provide evidence of a grouping with other <italic>Gracilacus</italic> and some species of <italic>Paratylenchus</italic> with stylet length of females longer than 41 μm deposited in GenBank.</p></abstract>

Gracilacus wuae n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet ranging from 80 to 93 μm long, the lip region not offset from the body contour, without lateral lips but with large and flat submedian lobes, the mouth opening slit-like elongated laterally and surrounded by lateral flaps, the excretory pore is anterior to the knobs of the stylet; males without stylet and the pharynx degenerated. The fourth-stage juveniles lack a stylet, the pharynx degenerated, and can be differentiated into preadult females and males based on the position of the genital primordia. The third-stage juveniles are similar to females but smaller. Phylogenetic studies using the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer (ITS) sequences collectively provide evidence of a grouping with other Gracilacus and some species of Paratylenchus with stylet length of females longer than 41 μm deposited in GenBank.

Morphological and Molecular Characterization of <italic>Gracilacus wuae</italic> n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (<italic>Heracleum maximum</italic>) in Ontario, Canada

North Carolina Department of Agriculture & Consumer Services

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological and Molecular Characterization of  n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip () in Ontario, Canada

Gracilacus wuae n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet...

Morphological and Molecular Characterization of Gracilacus wuae n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (Heracleum maximum) in Ontario, Canada

Published Online: Jul 21, 2017

Page range: 203 - 213

Received: May 05, 2016

DOI: https://doi.org/10.21307/jofnem-2017-028

Keywords
molecular, morphology, morphometric, new species, taxonomy

© 2016 Qing Yu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Morphological and Molecular Characterization of Gracilacus wuae n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (Heracleum maximum) in Ontario, Canada

Published Online: Jul 21, 2017

Page range: 203 - 213

Received: May 05, 2016

DOI: https://doi.org/10.21307/jofnem-2017-028

Keywordsmolecular, morphology, morphometric, new species, taxonomy

© 2016 Qing Yu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
molecular, morphology, morphometric, new species, taxonomy