<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A new species of entomopathogenic nematode (EPN), <italic>Steinernema biddulphi</italic> n. sp., was isolated from a maize field in Senekal, Free State Province of South Africa. Morphological and molecular studies indicated the distinctness of <italic>S. biddulphi</italic> n. sp. from other <italic>Steinernema</italic> species. <italic>Steinernema biddulphi</italic> n. sp. is characterized IJs with average body length of 663 μm (606–778 μm), lateral fields with six ridges in mid-body region forming the formula 2,6,2. Excretory pore located anterior to mid-pharynx (D% = 46). Hyaline layer occupies approximately half of tail length. Male spicules slightly to moderately curved, with a sharp tip and golden brown in color. The first generation of males lacking a mucron on the tail tip while the second generation males with a short filamentous mucron. Genital papillae with 11 pairs and one unpaired preanal papilla. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal DNA (rDNA). Phylogenetic data show that <italic>S. biddulphi</italic> n. sp. belongs to the “<italic>bicornutum</italic>” clade within the Steinernematidae family.</p></abstract>

A new species of entomopathogenic nematode (EPN), Steinernema biddulphi n. sp., was isolated from a maize field in Senekal, Free State Province of South Africa. Morphological and molecular studies indicated the distinctness of S. biddulphi n. sp. from other Steinernema species. Steinernema biddulphi n. sp. is characterized IJs with average body length of 663 μm (606–778 μm), lateral fields with six ridges in mid-body region forming the formula 2,6,2. Excretory pore located anterior to mid-pharynx (D% = 46). Hyaline layer occupies approximately half of tail length. Male spicules slightly to moderately curved, with a sharp tip and golden brown in color. The first generation of males lacking a mucron on the tail tip while the second generation males with a short filamentous mucron. Genital papillae with 11 pairs and one unpaired preanal papilla. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal DNA (rDNA). Phylogenetic data show that S. biddulphi n. sp. belongs to the “bicornutum” clade within the Steinernematidae family.

<italic>Steinernema biddulphi</italic> n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

 n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

A new species of entomopathogenic nematode (EPN), Steinernema biddulphi n. sp., was isolated from a maize field in Senekal, Free State Province of South...

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

Published Online: Jul 21, 2017

Page range: 148 - 158

Received: Feb 02, 2013

DOI: https://doi.org/10.21307/jofnem-2017-022

Keywords
D2-D3, entomopathogenic nematodes, ITS, new species, phylogeny, South Africa, taxonomy

© 2016 Harun Cimen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

Published Online: Jul 21, 2017

Page range: 148 - 158

Received: Feb 02, 2013

DOI: https://doi.org/10.21307/jofnem-2017-022

KeywordsD2-D3, entomopathogenic nematodes, ITS, new species, phylogeny, South Africa, taxonomy

© 2016 Harun Cimen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
D2-D3, entomopathogenic nematodes, ITS, new species, phylogeny, South Africa, taxonomy