<abstract xmlns="http://www.w3.org/1999/xhtml">Twenty-five red cell samples with a positive anti-IgG direct antiglobulin test (DAT) were tested with human monoclonal IgM Jka and Jkb antibodies. Red cell samples were first tested by a 5-minute incubation tube test with the monoclonal antibodies (MAbs). The same red cells were then chloroquine diphosphate (CDP)-treated, and retested. Eleven of the CDP-treated samples were also tested with conventional polyclonal antibodies (PAbs) that required a 37°C incubation for 30 minutes, followed by an indirect antiglobulin test. The Jka and Jkb MAbs consistently gave the same phenotype results both on untreated DAT-positive red cells and on the same cells after CDP treatment. Two of the CDP-treated samples had diminished antigen expression with the MAbs, a finding that may have been caused by the CDP treatment. One untreated sample, which spontaneously agglutinated in a low-protein medium, was incorrectly phenotyped with the anti-Jka MAb, but both MAbs and PAbs gave the same correct results with the CDP-treated cells. These findings illustrate that the use of Jka and Jkb MAbs in phenotyping DAT-positive specimens is practical and beneficial. <bold><italic>Immunohematology</italic></bold> 1994;10:16.</abstract>

Twenty-five red cell samples with a positive anti-IgG direct antiglobulin test (DAT) were tested with human monoclonal IgM Jka and Jkb antibodies. Red cell samples were first tested by a 5-minute incubation tube test with the monoclonal antibodies (MAbs). The same red cells were then chloroquine diphosphate (CDP)-treated, and retested. Eleven of the CDP-treated samples were also tested with conventional polyclonal antibodies (PAbs) that required a 37°C incubation for 30 minutes, followed by an indirect antiglobulin test. The Jka and Jkb MAbs consistently gave the same phenotype results both on untreated DAT-positive red cells and on the same cells after CDP treatment. Two of the CDP-treated samples had diminished antigen expression with the MAbs, a finding that may have been caused by the CDP treatment. One untreated sample, which spontaneously agglutinated in a low-protein medium, was incorrectly phenotyped with the anti-Jka MAb, but both MAbs and PAbs gave the same correct results with the CDP-treated cells. These findings illustrate that the use of Jka and Jkb MAbs in phenotyping DAT-positive specimens is practical and beneficial. Immunohematology 1994;10:16.

Use of monoclonal Jka and Jkb reagents in phenotyping red cells with a positive direct antiglobulin test

Immunohematology

Use of monoclonal Jk and Jk reagents in phenotyping red cells with a positive direct antiglobulin test

Twenty-five red cell samples with a positive anti-IgG direct antiglobulin test (DAT) were tested with human monoclonal IgM Jka and Jkb antibodies. Red cell...

Use of monoclonal Jk^a and Jk^b reagents in phenotyping red cells with a positive direct antiglobulin test

Published Online: Nov 22, 2020

Page range: 16 - 18

DOI: https://doi.org/10.21307/immunohematology-2019-813

© 1994 J. Brazell et al., published by Sciendo