<abstract xmlns="http://www.w3.org/1999/xhtml"><p>ABO discrepancy was noted when a patient’s unwashed saline red cell suspension was tested with monoclonal ABO reagents. The discrepancy was resolved when a washed (× 3) saline red cell suspension was used in repeat testing. The patient’s serum contained an autoagglutinin that reacted optimally below pH 7.0. Tire discrepancy occurred when monoclonal ABO reagents, formulated at a low pH, lowered the pH of the reaction mixture, and autoagglutination was observed. <bold><italic>Immunohematology</italic></bold> 1995;11:71–73.</p></abstract>

ABO discrepancy was noted when a patient’s unwashed saline red cell suspension was tested with monoclonal ABO reagents. The discrepancy was resolved when a washed (× 3) saline red cell suspension was used in repeat testing. The patient’s serum contained an autoagglutinin that reacted optimally below pH 7.0. Tire discrepancy occurred when monoclonal ABO reagents, formulated at a low pH, lowered the pH of the reaction mixture, and autoagglutination was observed. Immunohematology 1995;11:71–73.

ABO discrepancy with monoclonal ABO reagents caused by a pH-dependent autoantibody

Immunohematology

ABO discrepancy was noted when a patient’s unwashed saline red cell suspension was tested with monoclonal ABO reagents. The discrepancy was resolved when a...

ABO discrepancy with monoclonal ABO reagents caused by a pH-dependent autoantibody

Published Online: Nov 17, 2020

Page range: 71 - 73

DOI: https://doi.org/10.21307/immunohematology-2019-792

© 1995 M. S. Kennedy et al., published by Sciendo