<abstract xmlns="http://www.w3.org/1999/xhtml">The Cromer blood group antigens reside on the complement regulatory protein, decay-accelerating factor (DAF). The Cromer system comprises 10 antigens, 3 of which are of low incidence. When an individual is homozygous for the allele encoding one of these low-incidence antigens, they are liable to produce an antibody to the antithetical high-frequency antigen if challenged by pregnancy or transfusion. These antibodies are often difficult to identify, because of the lack of readily available antigen-negative cells and typing sera. In blacks, about 5 percent of individuals carry the rare <italic>Tcb</italic> Cromer allele. We have shown that the presence of the low-incidence <italic>Tcb</italic> allele can be detected by polymerase chain reaction (PCR) amplification of a fragment of the gene encoding DAF, followed by allelespecific restriction enzyme digestion.</abstract>

The Cromer blood group antigens reside on the complement regulatory protein, decay-accelerating factor (DAF). The Cromer system comprises 10 antigens, 3 of which are of low incidence. When an individual is homozygous for the allele encoding one of these low-incidence antigens, they are liable to produce an antibody to the antithetical high-frequency antigen if challenged by pregnancy or transfusion. These antibodies are often difficult to identify, because of the lack of readily available antigen-negative cells and typing sera. In blacks, about 5 percent of individuals carry the rare Tcb Cromer allele. We have shown that the presence of the low-incidence Tcb allele can be detected by polymerase chain reaction (PCR) amplification of a fragment of the gene encoding DAF, followed by allelespecific restriction enzyme digestion.

Identification of the <italic>Tcb</italic> allele of the Cromer blood group gene by PCR and RFLP analysis

Immunohematology

Identification of the  allele of the Cromer blood group gene by PCR and RFLP analysis

The Cromer blood group antigens reside on the complement regulatory protein, decay-accelerating factor (DAF). The Cromer system comprises 10 antigens, 3 of...

Identification of the Tc^b allele of the Cromer blood group gene by PCR and RFLP analysis

Published Online: Nov 17, 2020

Page range: 1 - 4

DOI: https://doi.org/10.21307/immunohematology-2019-776

© 1995 M. N. Udani et al., published by Sciendo

Identification of the Tcb allele of the Cromer blood group gene by PCR and RFLP analysis

Published Online: Nov 17, 2020

Page range: 1 - 4

DOI: https://doi.org/10.21307/immunohematology-2019-776

© 1995 M. N. Udani et al., published by Sciendo

Identification of the Tc^b allele of the Cromer blood group gene by PCR and RFLP analysis