<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A recently introduced system for antibody detection (ReACT™) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT™ system to a conventional tube low-ionic-strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sd<sup>a</sup>, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method. <bold><italic>Immunohematology</italic> 1999;15:75–77</bold>.</p></abstract>

A recently introduced system for antibody detection (ReACT™) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT™ system to a conventional tube low-ionic-strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method. Immunohematology 1999;15:75–77.

A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

Immunohematology

A recently introduced system for antibody detection (ReACT™) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We...

A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

Published Online: Oct 26, 2020

Page range: 75 - 77

DOI: https://doi.org/10.21307/immunohematology-2019-618

© 1999 R.S. Shirey et al., published by Sciendo.