<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Despite the wide use of the antibody detection test for unexpected antibodies, controversy still remains regarding the use of enzyme-treated red blood cells. Over a 6-year period, 72,573 samples from 49,863 patients submitted for pretransfusion compatibility testing were examined for unexpected antibodies. The antibody detection tests included a low-ionic-strength solution (LISS) indirect antiglobulin test and a two-stage papain (2SP) test. One thousand and seventy of the 2267 (47%) antibodies tested by 2SP were reactive only by the 2SP test. Overall, the 2SP test detected only 0.6% of antibodies considered to be clinically significant (10 examples of anti-c and 2 examples of anti-e). The slight additional safety provided by detection of clinically-significant antibodies is overshadowed by the high number of clinically-insignificant antibodies detected by the 2SP test. <bold><italic>Immunohematology</italic> 2001;17:122–124</bold>.</p></abstract>

Despite the wide use of the antibody detection test for unexpected antibodies, controversy still remains regarding the use of enzyme-treated red blood cells. Over a 6-year period, 72,573 samples from 49,863 patients submitted for pretransfusion compatibility testing were examined for unexpected antibodies. The antibody detection tests included a low-ionic-strength solution (LISS) indirect antiglobulin test and a two-stage papain (2SP) test. One thousand and seventy of the 2267 (47%) antibodies tested by 2SP were reactive only by the 2SP test. Overall, the 2SP test detected only 0.6% of antibodies considered to be clinically significant (10 examples of anti-c and 2 examples of anti-e). The slight additional safety provided by detection of clinically-significant antibodies is overshadowed by the high number of clinically-insignificant antibodies detected by the 2SP test. Immunohematology 2001;17:122–124.

One thousand seventy antibodies detected only by a 2-stage papain test: wanted and unwanted positive reactions

Immunohematology

Despite the wide use of the antibody detection test for unexpected antibodies, controversy still remains regarding the use of enzyme-treated red blood cells....

One thousand seventy antibodies detected only by a 2-stage papain test: wanted and unwanted positive reactions

Published Online: Oct 14, 2020

Page range: 122 - 124

DOI: https://doi.org/10.21307/immunohematology-2019-565

Keywords
antibody detection, enzyme-only antibodies

© 2019 D. CastellÁ et al., published by Sciendo

One thousand seventy antibodies detected only by a 2-stage papain test: wanted and unwanted positive reactions

Published Online: Oct 14, 2020

Page range: 122 - 124

DOI: https://doi.org/10.21307/immunohematology-2019-565

Keywordsantibody detection, enzyme-only antibodies

© 2019 D. CastellÁ et al., published by Sciendo

Keywords
antibody detection, enzyme-only antibodies