<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Treatment of RBCs with protease enzymes or dithiothreitol (DTT) causes denaturation of several RBC antigens and is regularly used in antibody identification. In this study, we have standardized enzyme and DTT treatment of adherent RBCs in the magnetic-mixed passive hemagglutination assay (M-MPHA) for antibody identification. We have also tried drying these treated RBCs. The optimal enzyme and DTT treatment conditions for intact adherent RBCs were determined, in addition to the optimal condition for drying RBCs. All tested agents with the exception of chymotrypsin could be applied to RBC drying,but the optimal condition for drying was different from that for intact RBCs. Enzyme or DTT treatment of adherent RBCs and their application in M-MPHA provide a simple and convenient method for antibody identification. Furthermore, the technique of drying treated RBCs provides an even stronger antibody identification tool, since dried RBCs can be stored for a long period. <bold><italic>Immunohematology</italic> 2002;18:114–119.</bold></p></abstract>

Treatment of RBCs with protease enzymes or dithiothreitol (DTT) causes denaturation of several RBC antigens and is regularly used in antibody identification. In this study, we have standardized enzyme and DTT treatment of adherent RBCs in the magnetic-mixed passive hemagglutination assay (M-MPHA) for antibody identification. We have also tried drying these treated RBCs. The optimal enzyme and DTT treatment conditions for intact adherent RBCs were determined, in addition to the optimal condition for drying RBCs. All tested agents with the exception of chymotrypsin could be applied to RBC drying,but the optimal condition for drying was different from that for intact RBCs. Enzyme or DTT treatment of adherent RBCs and their application in M-MPHA provide a simple and convenient method for antibody identification. Furthermore, the technique of drying treated RBCs provides an even stronger antibody identification tool, since dried RBCs can be stored for a long period. Immunohematology 2002;18:114–119.

Enzyme and DTT treatment of adherent RBCs for antibody identification by a solid phase immunoassay system

Olympus Optical Co., Ltd, Reagent Development

Shuwa-Shibapark, Building B, 4-1, Shibakouen 2, Minato-ku

Immunohematology

Treatment of RBCs with protease enzymes or dithiothreitol (DTT) causes denaturation of several RBC antigens and is regularly used in antibody identification....

Enzyme and DTT treatment of adherent RBCs for antibody identification by a solid phase immunoassay system

Published Online: Oct 14, 2020

Page range: 114 - 119

DOI: https://doi.org/10.21307/immunohematology-2019-527

Keywords
RBC treatment, solid-phase, M-MPHA, irregular antibodies, antibody identification, enzyme, DTT, RBC drying

© 2002 T. Tamai et al., published by Sciendo

Enzyme and DTT treatment of adherent RBCs for antibody identification by a solid phase immunoassay system

Published Online: Oct 14, 2020

Page range: 114 - 119

DOI: https://doi.org/10.21307/immunohematology-2019-527

KeywordsRBC treatment, solid-phase, M-MPHA, irregular antibodies, antibody identification, enzyme, DTT, RBC drying

© 2002 T. Tamai et al., published by Sciendo

Keywords
RBC treatment, solid-phase, M-MPHA, irregular antibodies, antibody identification, enzyme, DTT, RBC drying