<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In two to five percent of cases of autoimmune hemolytic anemia (AIHA), the patient’s RBCs are negative in the direct antiglobulin test (DAT). We describe a patient with AIHA with a negative DAT when tested by the traditional tube test but with a strongly positive one when tested by column agglutination. When the DAT was repeated by tube test using 4°C saline washes, it became positive. This phenomenon has been observed when low-affinity antibodies are involved in AIHA. The patient’s history and serologic findings are detailed in this report. <bold><italic>Immunohematology</italic> 2002;18:109–113.</bold></p></abstract>

In two to five percent of cases of autoimmune hemolytic anemia (AIHA), the patient’s RBCs are negative in the direct antiglobulin test (DAT). We describe a patient with AIHA with a negative DAT when tested by the traditional tube test but with a strongly positive one when tested by column agglutination. When the DAT was repeated by tube test using 4°C saline washes, it became positive. This phenomenon has been observed when low-affinity antibodies are involved in AIHA. The patient’s history and serologic findings are detailed in this report. Immunohematology 2002;18:109–113.

Clinically significant autoimmune hemolytic anemia with a negative direct antiglobulin test by routine tube test and positive by column agglutination method

Immunohematology

In two to five percent of cases of autoimmune hemolytic anemia (AIHA), the patient’s RBCs are negative in the direct antiglobulin test (DAT). We describe a...

Clinically significant autoimmune hemolytic anemia with a negative direct antiglobulin test by routine tube test and positive by column agglutination method

Published Online: Oct 14, 2020

Page range: 109 - 113

DOI: https://doi.org/10.21307/immunohematology-2019-526

Keywords
direct antiglobulin test, column agglutination method, autoimmune hemolytic anemia, low-affinity antibodies

© 2002 M. Lai et al., published by Sciendo

Clinically significant autoimmune hemolytic anemia with a negative direct antiglobulin test by routine tube test and positive by column agglutination method

Published Online: Oct 14, 2020

Page range: 109 - 113

DOI: https://doi.org/10.21307/immunohematology-2019-526

Keywordsdirect antiglobulin test, column agglutination method, autoimmune hemolytic anemia, low-affinity antibodies

© 2002 M. Lai et al., published by Sciendo

Keywords
direct antiglobulin test, column agglutination method, autoimmune hemolytic anemia, low-affinity antibodies