<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Pretransfusion tests must detect antibodies that can shorten the life of red blood cells (RBCs). Some studies have demonstrated the existence of clinically significant antibodies detected at 37°C in saline that are not detected by the indirect antiglobulin test (IAT) when the conventional tube test is used. Our aim was to determine whether these antibodies, detected with a 37°C saline tube test, are also detected when a sensitive column gel agglutination method is used. The 2373 pretransfusion samples were tested as they were received (from May 1998 to December 1999), in 37°C saline and by IAT using the DiaMed gel system. The screening tests were performed using 50 <italic>μ</italic>L of 0.8% low-ionic-strength saline suspended RBCs and 50 <italic>μ</italic>L of plasma. The tests were examined for agglutination and hemolysis. Two hundred and thirty three samples (9.81%) were reactive by IAT and 88 (3.70%) by 37°C saline. All 88 samples reactive by 37°C saline also reacted by IAT. These data indicate that 37°C saline is not an essential pretransfusion procedure when the DiaMed gel test is used. <bold><italic>Immunohematology</italic> 2002;18:13–15</bold>.</p></abstract>

Pretransfusion tests must detect antibodies that can shorten the life of red blood cells (RBCs). Some studies have demonstrated the existence of clinically significant antibodies detected at 37°C in saline that are not detected by the indirect antiglobulin test (IAT) when the conventional tube test is used. Our aim was to determine whether these antibodies, detected with a 37°C saline tube test, are also detected when a sensitive column gel agglutination method is used. The 2373 pretransfusion samples were tested as they were received (from May 1998 to December 1999), in 37°C saline and by IAT using the DiaMed gel system. The screening tests were performed using 50 μL of 0.8% low-ionic-strength saline suspended RBCs and 50 μL of plasma. The tests were examined for agglutination and hemolysis. Two hundred and thirty three samples (9.81%) were reactive by IAT and 88 (3.70%) by 37°C saline. All 88 samples reactive by 37°C saline also reacted by IAT. These data indicate that 37°C saline is not an essential pretransfusion procedure when the DiaMed gel test is used. Immunohematology 2002;18:13–15.

Antibody screening in 37°C saline. Is it safe to omit it using the indirect antiglobulin (gel) test?

Immunohematology

Pretransfusion tests must detect antibodies that can shorten the life of red blood cells (RBCs). Some studies have demonstrated the existence of clinically...

Antibody screening in 37°C saline. Is it safe to omit it using the indirect antiglobulin (gel) test?

Published Online: Oct 14, 2020

Page range: 13 - 15

DOI: https://doi.org/10.21307/immunohematology-2019-498

Keywords
antibody screening, pretransfusion tests, gel test

© 2002 J. Duran et al., published by Sciendo

Antibody screening in 37°C saline. Is it safe to omit it using the indirect antiglobulin (gel) test?

Published Online: Oct 14, 2020

Page range: 13 - 15

DOI: https://doi.org/10.21307/immunohematology-2019-498

Keywordsantibody screening, pretransfusion tests, gel test

© 2002 J. Duran et al., published by Sciendo

Keywords
antibody screening, pretransfusion tests, gel test