<abstract xmlns="http://www.w3.org/1999/xhtml">We compared the results of routine blood tests for 102 blood donors’ samples and 100 patients’ samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray- dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors’ samples included time delayed antigen testing and antibody identification and half-draw/half- evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients’ samples collected in spray-dried K2EDTA and spray-dried K3EDTA and for an additional 50 patients’ samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients’ samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors’ and patients’ samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.</abstract>

We compared the results of routine blood tests for 102 blood donors’ samples and 100 patients’ samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes to evaluate the impact of changes in formulation of the anticoagulant (K2EDTA vs. K3EDTA), its application (liquid vs. spray- dried), and tube material (glass vs. plastic). Methods for ABO/D testing, antibody screening, and antibody identification included direct hemagglutination/microplate (Olympus® PK 7200) and gel column methods (Ortho ID-Micro Typing System™/Gel Test™). Additional studies on blood donors’ samples included time delayed antigen testing and antibody identification and half-draw/half- evacuated collections. Also, we compared the results of routine ABO/D testing and antibody screening for 50 patients’ samples collected in spray-dried K2EDTA and spray-dried K3EDTA and for an additional 50 patients’ samples collected in spray-dried K2EDTA tubes from two different manufacturers. All patients’ samples were tested in parallel by solid phase/microplate method (Immucor® ABS 2000) and the standard manual tube method. All test results for routine blood bank tests on donors’ and patients’ samples were concordant, demonstrating the equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA blood collection tubes for routine donor center or transfusion service testing.

Equivalence of spray-dried K2EDTA, spray-dried K3EDTA, and liquid K3EDTA anticoagulated blood samples for routine blood center or transfusion service testing

Gulf Coast Regional Blood Center Houston, TX

Immunohematology

Equivalence of spray-dried KEDTA, spray-dried KEDTA, and liquid KEDTA anticoagulated blood samples for routine blood center or transfusion service testing

We compared the results of routine blood tests for 102 blood donors’ samples and 100 patients’ samples collected in spray-dried K2EDTA, spray-dried K3EDTA, and...

Equivalence of spray-dried K₂EDTA, spray-dried K₃EDTA, and liquid K₃EDTA anticoagulated blood samples for routine blood center or transfusion service testing

Published Online: Oct 14, 2020

Page range: 117 - 121

DOI: https://doi.org/10.21307/immunohematology-2019-491

Keywords
blood typing, antibody screening, blood donors, EDTA, blood collection tubes

© 2003 S. Leathem et al., published by Sciendo