<abstract xmlns="http://www.w3.org/1999/xhtml">Previous studies have shown that RBCs with residual WBCs stored in LISS and neomycin sulfate develop characteristics associated with enzyme-treated RBCs. During a mass screening program to antigen type donor RBCs, we observed that the Fya antigens on a RBC sample from an in-house panel became non-detectable with anti-Fya after incubation overnight in Diluent 2 from Micro Typing Systems, Inc. (MTS, Pompano Beach, FL). In response to this observation, we initiated an investigation to determine the cause. Tests were performed according to the manfacturer’s instructions in MTS neutral gel cards or gel cards containing anti-IgG. We found that a reduction or loss of the Fya, Fyb, and M antigens occurs when RBCs were prepared from samples containing residual WBCs (as a source of enzymes) and subsequently incubated in media containing neomycin sulfate and LISS. We showed that the effect did not occur in the absence of neomycin sulfate. RBC antigens can be altered in LISS if they have first been exposed to neomycin. We recommend restricting the use of RBCs suspended in MTS Diluent 2 to the day of dilution (as indicated in the package insert) if preparing reagent RBCs from sources that were not leukoreduced and were stored in the presence of neomycin.</abstract>

Previous studies have shown that RBCs with residual WBCs stored in LISS and neomycin sulfate develop characteristics associated with enzyme-treated RBCs. During a mass screening program to antigen type donor RBCs, we observed that the Fya antigens on a RBC sample from an in-house panel became non-detectable with anti-Fya after incubation overnight in Diluent 2 from Micro Typing Systems, Inc. (MTS, Pompano Beach, FL). In response to this observation, we initiated an investigation to determine the cause. Tests were performed according to the manfacturer’s instructions in MTS neutral gel cards or gel cards containing anti-IgG. We found that a reduction or loss of the Fya, Fyb, and M antigens occurs when RBCs were prepared from samples containing residual WBCs (as a source of enzymes) and subsequently incubated in media containing neomycin sulfate and LISS. We showed that the effect did not occur in the absence of neomycin sulfate. RBC antigens can be altered in LISS if they have first been exposed to neomycin. We recommend restricting the use of RBCs suspended in MTS Diluent 2 to the day of dilution (as indicated in the package insert) if preparing reagent RBCs from sources that were not leukoreduced and were stored in the presence of neomycin.

Loss of enzyme-sensitive antigens due to the presence of leukocytes, neomycin sulfate, and LISS

Immunohematology

Previous studies have shown that RBCs with residual WBCs stored in LISS and neomycin sulfate develop characteristics associated with enzyme-treated RBCs....

Loss of enzyme-sensitive antigens due to the presence of leukocytes, neomycin sulfate, and LISS

Published Online: Oct 14, 2020

Page range: 109 - 111

DOI: https://doi.org/10.21307/immunohematology-2019-489

Keywords
blood group antigens, enzyme-sensitive antigens, protease-sensitive, RBCs

© 2003 R.W. Velliquette et al., published by Sciendo

Loss of enzyme-sensitive antigens due to the presence of leukocytes, neomycin sulfate, and LISS

Published Online: Oct 14, 2020

Page range: 109 - 111

DOI: https://doi.org/10.21307/immunohematology-2019-489

Keywordsblood group antigens, enzyme-sensitive antigens, protease-sensitive, RBCs

© 2003 R.W. Velliquette et al., published by Sciendo

Keywords
blood group antigens, enzyme-sensitive antigens, protease-sensitive, RBCs