<abstract xmlns="http://www.w3.org/1999/xhtml">Two transfused Caucasian patients presented with possible delayed transfusion reactions. Both patients demonstrated an anti-Fya (Fy1) plus anti-Fyb (Fy2) pattern of reactivity in their sera. The patients’ red blood cells (RBCs) were Fy:1,–2,3. Both had positive direct antiglobulin tests (DAT) with anti-IgG and -C3d. The serum antibodies reacted with the patients’ RBCs drawn when the DATs were negative Both patients’ serum samples showed reactivity with Fy:1,–2 (1 +), Fy:–1,2 (3+), Fy:1,2 (3+) RBCs at the antiglobulin phase (ACT) but were nonreactive with Fy: –1, –2 RBCs. The anti-bodies in both cases were nonreactive with papain- or ficin-treated RBCs -and reacted with Rhnull RBCs, which ruled out anti-Fy3 and anti-Fy5, respectively. These cases suggest mimicking autoantibody specificities in the Duffy system. One case may indicate that the degree of reactivity is dependent on the Duffy phenotype of the RBCs tested (Fy: –1,2 > Fy:1,2 > Fy: –1, – 2). An unusual characteristic seen in both of these cases was hemolysis in the EDTA cell samples but none in the clotted samples.</abstract>

Two transfused Caucasian patients presented with possible delayed transfusion reactions. Both patients demonstrated an anti-Fya (Fy1) plus anti-Fyb (Fy2) pattern of reactivity in their sera. The patients’ red blood cells (RBCs) were Fy:1,–2,3. Both had positive direct antiglobulin tests (DAT) with anti-IgG and -C3d. The serum antibodies reacted with the patients’ RBCs drawn when the DATs were negative Both patients’ serum samples showed reactivity with Fy:1,–2 (1 +), Fy:–1,2 (3+), Fy:1,2 (3+) RBCs at the antiglobulin phase (ACT) but were nonreactive with Fy: –1, –2 RBCs. The anti-bodies in both cases were nonreactive with papain- or ficin-treated RBCs -and reacted with Rhnull RBCs, which ruled out anti-Fy3 and anti-Fy5, respectively. These cases suggest mimicking autoantibody specificities in the Duffy system. One case may indicate that the degree of reactivity is dependent on the Duffy phenotype of the RBCs tested (Fy: –1,2 > Fy:1,2 > Fy: –1, – 2). An unusual characteristic seen in both of these cases was hemolysis in the EDTA cell samples but none in the clotted samples.

Two cases of autoantibodies that demonstrate mimicking specificity in the Duffy blood group system

Immunohematology

Two transfused Caucasian patients presented with possible delayed transfusion reactions. Both patients demonstrated an anti-Fya (Fy1) plus anti-Fyb (Fy2)...

Two cases of autoantibodies that demonstrate mimicking specificity in the Duffy blood group system

Published Online: Dec 20, 2020

Page range: 87 - 91

DOI: https://doi.org/10.21307/immunohematology-2019-1050

© 1990 T. Harris et al., published by Sciendo.