<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Reticulocytes can be separated from more mature red blood cells based on differences in density. A method for obtaining autologous reticulocytes in ethylenediaminetetraacetic acid (EDTA) whole blood samples containing both autologous and transfused cells uses a microhematocrit centrifuge. The less dense reticulocytes harvested from the top 5 mm of microhematocrit tubes can be used to determine the patient’s phenotype or assess whether a transfusion reaction is taking place. This method can be performed using equipment, reagents, and supplies readily available in most laboratories. <italic><bold>Immunohematology</bold></italic><bold>2015;31:152–154.</bold></p></abstract>

Reticulocytes can be separated from more mature red blood cells based on differences in density. A method for obtaining autologous reticulocytes in ethylenediaminetetraacetic acid (EDTA) whole blood samples containing both autologous and transfused cells uses a microhematocrit centrifuge. The less dense reticulocytes harvested from the top 5 mm of microhematocrit tubes can be used to determine the patient’s phenotype or assess whether a transfusion reaction is taking place. This method can be performed using equipment, reagents, and supplies readily available in most laboratories. Immunohematology2015;31:152–154.

Recovery of autologous reticulocytes by microhematocrit cell separation

Immunohematology

{"article-title":"Recovery of autologous reticulocytes by microhematocrit cell separation"}

Reticulocytes can be separated from more mature red blood cells based on differences in density. A method for obtaining autologous reticulocytes in...

Recovery of autologous reticulocytes by microhematocrit cell separation

Published Online: Oct 26, 2019

Page range: 152 - 154

DOI: https://doi.org/10.21307/immunohematology-2019-084

Keywords
Microhematocrit centrifugation, micro-hematocrit cell separation, cell separation method, autologous reticulocytes, reticulocyte separation, autologous red cell recovery

© 2015 C.W. Hall et al., published by Sciendo

Recovery of autologous reticulocytes by microhematocrit cell separation

Published Online: Oct 26, 2019

Page range: 152 - 154

DOI: https://doi.org/10.21307/immunohematology-2019-084

KeywordsMicrohematocrit centrifugation, micro-hematocrit cell separation, cell separation method, autologous reticulocytes, reticulocyte separation, autologous red cell recovery

© 2015 C.W. Hall et al., published by Sciendo

Keywords
Microhematocrit centrifugation, micro-hematocrit cell separation, cell separation method, autologous reticulocytes, reticulocyte separation, autologous red cell recovery