<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This paper investigates phage-coated magnetoelastic (ME) biosentinels that capture and detect low-concentration pathogenic bacteria in stagnant liquid. These biosentinels are composed of a freestanding ME resonator platform coated with a landscape phage that specifically binds with the pathogens of interest. These biosentinels can be moved through a liquid by externally applied magnetic fields. When a time-varying magnetic field is applied, the ME biosentinels can be placed into mechanical resonance by magnetostriction. As soon as the biosentinels bind with the target pathogen through the phagebased biomolecular recognition, a change in the biosentinel’s resonant frequency occurs, and thereby the presence of the target pathogen can be detected. Detection of <italic>Bacillus anthracis</italic> spores under stagnant flow conditions was demonstrated.</p>
</abstract>

This paper investigates phage-coated magnetoelastic (ME) biosentinels that capture and detect low-concentration pathogenic bacteria in stagnant liquid. These biosentinels are composed of a freestanding ME resonator platform coated with a landscape phage that specifically binds with the pathogens of interest. These biosentinels can be moved through a liquid by externally applied magnetic fields. When a time-varying magnetic field is applied, the ME biosentinels can be placed into mechanical resonance by magnetostriction. As soon as the biosentinels bind with the target pathogen through the phagebased biomolecular recognition, a change in the biosentinel’s resonant frequency occurs, and thereby the presence of the target pathogen can be detected. Detection of Bacillus anthracis spores under stagnant flow conditions was demonstrated.

Magnetoelastic Biosentinels for the Capture and Detection of Low-Concentration Pathogens in Liquid

International Journal on Smart Sensing and Intelligent Systems

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This paper investigates phage-coated magnetoelastic (ME) biosentinels that capture and detect low-concentration pathogenic bacteria in stagnant liquid. These...

Magnetoelastic Biosentinels for the Capture and Detection of Low-Concentration Pathogens in Liquid

Published Online: Feb 15, 2020

Page range: 1 - 4

DOI: https://doi.org/10.21307/ijssis-2019-075

Keywords
biosentinel, phage, detection, pathogen

© 2019 Authors., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Magnetoelastic Biosentinels for the Capture and Detection of Low-Concentration Pathogens in Liquid

Published Online: Feb 15, 2020

Page range: 1 - 4

DOI: https://doi.org/10.21307/ijssis-2019-075

Keywordsbiosentinel, phage, detection, pathogen

© 2019 Authors., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
biosentinel, phage, detection, pathogen