Effect of combined oral contraceptives on orthodontic tooth movement in a female rat model
Published Online: Jul 20, 2021
Page range: 127 - 133
Received: Dec 01, 2018
Accepted: May 01, 2019
DOI: https://doi.org/10.21307/aoj-2020-041
© 2019 Jinghong Yu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
To investigate the effect of combined oral contraceptives (COC) on orthodontic tooth movement (OTM) and periodontal remodelling in a female rat model.
Methods
A total of 80 three-month-old female, Sprague-Dawley rats were randomly divided into experimental and control groups. The maxillary first molars were moved mesially using nickel-titanium coil springs (50 g force). The experimental group (N = 40) ingested 1.5 ml COC (Marvelon, 0.12 mg/d, N.V. Organon, Oss, The Netherlands) daily. The control group (N = 40) ingested 1.5 ml saline (0.9% sodium chloride) daily. After 7, 14, 21 and 28 days of force application, 10 rats in each group were euthanased and a vernier calliper was used to measure the orthodontic movement of the first molar. Root resorption at pressure areas was assessed by H and E staining. Micro-CT was used to detect alveolar bone mineral density.
Results and conclusion
The amount of OTM in the experimental group (0.46 ± 0.16 mm) was significantly less than in the control group (0.85 ± 0.25 mm;