<abstract xmlns="http://www.w3.org/1999/xhtml">

Commonly used synthetic dyes cause serious problems with their efficient removal from sewage. The bioaugmentation of sewage treatment systems with highly decolorizing bacteria may be a solution. The aim of the study was the screening of bacteria with high ability to remove synthetic dyes (brilliant green (BG), crystal violet (CV), erythrosine (Er). The bacteria were isolated from municipal sewage, compost and rotten beech wood. Mineral and nutrient solid growth media supplemented with dyes (BG or EB) at a concentration 0.1 gL-1 were used. At second stage of screening the liquid nutrient broth supplemented with one of dye (BG, CV or Er at concentration 0.1 gL-1) was used. The contents of dyes in samples (after 96 h) were measured spectrophotometrically. The largest number of decolorizers were obtained from wastewater, then from compost and the rotten wood. In the case of BG and CV even small differences in the structure of the molecules affect the results of dyes removal. The structurally simpler BG was definitely better removed than CV. The results of the removal of Er were worse than BG but better than CV. Bacteria isolated at mineral medium removed dyes with higher efficiency.
</abstract>

Commonly used synthetic dyes cause serious problems with their efficient removal from sewage. The bioaugmentation of sewage treatment systems with highly decolorizing bacteria may be a solution. The aim of the study was the screening of bacteria with high ability to remove synthetic dyes (brilliant green (BG), crystal violet (CV), erythrosine (Er). The bacteria were isolated from municipal sewage, compost and rotten beech wood. Mineral and nutrient solid growth media supplemented with dyes (BG or EB) at a concentration 0.1 gL-1 were used. At second stage of screening the liquid nutrient broth supplemented with one of dye (BG, CV or Er at concentration 0.1 gL-1) was used. The contents of dyes in samples (after 96 h) were measured spectrophotometrically. The largest number of decolorizers were obtained from wastewater, then from compost and the rotten wood. In the case of BG and CV even small differences in the structure of the molecules affect the results of dyes removal. The structurally simpler BG was definitely better removed than CV. The results of the removal of Er were worse than BG but better than CV. Bacteria isolated at mineral medium removed dyes with higher efficiency.

Isolation and Screening of Bacteria with Ability to Decolorize Selected Synthetic Dyes – Preliminary Results

Architecture, Civil Engineering, Environment

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Commonly used synthetic dyes cause serious problems with their efficient removal from sewage. The bioaugmentation of sewage treatment systems with highly...

Triphenylmethane dyes

Molecular formula: C₂₇H₃₄N₂O₄S	Molecular weight: 482.63 g/mol	Absorbance – λ max.[nm]: 624	Nr C.I.: 42040

Molecular formula: C₂₅H₃₀N₃Cl	Molecular weight: 407.98 g/mol	Absorbance – λ max.[nm]: 590	Nr C.I.: 42555
Fluorone dyes

Molecular formula: C₂₀H₆I₄Na₂O₅	Molecular weight: 879.84 g/mol	Absorbance – λ max.[nm]: 527	Nr C.I.: 45430
Disazo dyes

Molecular formula: C₃₄H₂₄N₆Na₄O₁₄S₄	Molecular weight 960.79 g/mol	Absorbance – λ max.[nm]: 606	Nr C.I.: 23860

Isolation and Screening of Bacteria with Ability to Decolorize Selected Synthetic Dyes – Preliminary Results

Published Online: Jul 21, 2020

Page range: 75 - 86

Received: Jan 31, 2020

Accepted: Apr 22, 2020

DOI: https://doi.org/10.21307/acee-2020-019

Keywords
Bacteria, Brilliant green, Crystal violet, Decolorization, Erythrosine, Fluorone dyes, Triphenylmethane dyes, Screening, Synthetic dyes.

© 2020 Ewa Zabłocka-Godlewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

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Figure 4.

Figure 5.

Figure 6.

Figure 7.

Figure 8.

Figure 9.

Dyes used at the experiment – characterization (based on [29])

Isolation and Screening of Bacteria with Ability to Decolorize Selected Synthetic Dyes – Preliminary Results

Published Online: Jul 21, 2020

Page range: 75 - 86

Received: Jan 31, 2020

Accepted: Apr 22, 2020

DOI: https://doi.org/10.21307/acee-2020-019

KeywordsBacteria, Brilliant green, Crystal violet, Decolorization, Erythrosine, Fluorone dyes, Triphenylmethane dyes, Screening, Synthetic dyes.

© 2020 Ewa Zabłocka-Godlewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

Figure 7.

Figure 8.

Figure 9.

Dyes used at the experiment – characterization (based on [29])

Keywords
Bacteria, Brilliant green, Crystal violet, Decolorization, Erythrosine, Fluorone dyes, Triphenylmethane dyes, Screening, Synthetic dyes.