<abstract xmlns="http://www.w3.org/1999/xhtml">

<bold>Introduction</bold> : The effect of low-level laser therapy on the secretion of tissue-type plasminogen activator (t-PA) and its inhibitor (PAI-1) in an endothelial cell (EC) culture under hyperglycemia is the subject of the presented work.
Hyperglycemia associated with diabetes causes vascular EC dysfunction. Low-level laser therapy is a good method to support the pharmacological treatment of diabetes complications.
<bold>Materials and methods</bold> : We used lasers of 2 wavelengths: 635 nm and 830 nm with dose of 2 J/cm2. The experiment was performed <italic>in vitro</italic> in 4 groups of EC: 1 – no glucose in culture medium, no irradiation (control), 2 – glucose, no irradiation, 3 – glucose, laser 635 nm, 4 – glucose, laser 830 nm. After 2 irradiations, cells were counted and the t-PA and PAI-1 antigen (Ag) concentration in the supernatant was measured.
<bold>Results</bold> : In group 2, we observed a statistically significant lower number of cells (p &lt; 0.0001) and a higher concentration of t-PA:Ag and PAI-1:Ag (p &lt; 0.05) compared to the control group. However, in groups 3 and 4, the number of cells increased and the concentration of t-PA:Ag and PAI-1:Ag decreased compared to group 2 and nearly reached the values in the control group.
<bold>Conclusions</bold> : Hyperglycemia affects the fibrinolytic activity of ECs which is manifested by a significant increase in t-PA:Ag and PAI:Ag concentrations – recognized markers of endothelial damage. Irradiation of ECs by a low-power laser caused attenuation of the adverse effects of hyperglycemia. A tendency towards a decrease in t-PA:Ag and PAI-1:Ag concentration in the super-natant was observed with a significant increase in the number of cells to values close to control.
</abstract>

Introduction : The effect of low-level laser therapy on the secretion of tissue-type plasminogen activator (t-PA) and its inhibitor (PAI-1) in an endothelial cell (EC) culture under hyperglycemia is the subject of the presented work.
Hyperglycemia associated with diabetes causes vascular EC dysfunction. Low-level laser therapy is a good method to support the pharmacological treatment of diabetes complications.
Materials and methods : We used lasers of 2 wavelengths: 635 nm and 830 nm with dose of 2 J/cm2. The experiment was performed in vitro in 4 groups of EC: 1 – no glucose in culture medium, no irradiation (control), 2 – glucose, no irradiation, 3 – glucose, laser 635 nm, 4 – glucose, laser 830 nm. After 2 irradiations, cells were counted and the t-PA and PAI-1 antigen (Ag) concentration in the supernatant was measured.
Results : In group 2, we observed a statistically significant lower number of cells (p < 0.0001) and a higher concentration of t-PA:Ag and PAI-1:Ag (p < 0.05) compared to the control group. However, in groups 3 and 4, the number of cells increased and the concentration of t-PA:Ag and PAI-1:Ag decreased compared to group 2 and nearly reached the values in the control group.
Conclusions : Hyperglycemia affects the fibrinolytic activity of ECs which is manifested by a significant increase in t-PA:Ag and PAI:Ag concentrations – recognized markers of endothelial damage. Irradiation of ECs by a low-power laser caused attenuation of the adverse effects of hyperglycemia. A tendency towards a decrease in t-PA:Ag and PAI-1:Ag concentration in the super-natant was observed with a significant increase in the number of cells to values close to control.

Can laser therapy modify the secretion of the tissue-type plasminogen activator and its inhibitor in an endothelial cell culture under hyperglycemia?

Collegium Medicum in Bydgoszcz, Department of Pathophysiology

Collegium Medicum in Bydgoszcz, Department of Physiotherapy

Pomeranian Medical University in Szczecin

Department of Normal and Clinical Anatomy

Pomeranian Journal of Life Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction : The effect of low-level laser therapy on the secretion of tissue-type plasminogen activator (t-PA) and its inhibitor (PAI-1) in an endothelial...

Can laser therapy modify the secretion of the tissue-type plasminogen activator and its inhibitor in an endothelial cell culture under hyperglycemia?

Published Online: Jun 24, 2021

Page range: 1 - 5

DOI: https://doi.org/10.21164/pomjlifesci.728

Keywords
low-level laser therapy, endothelial cell culture, t-PA, PAI-1, hyperglycemia

© 2021 Krzysztof Góralczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Can laser therapy modify the secretion of the tissue-type plasminogen activator and its inhibitor in an endothelial cell culture under hyperglycemia?

Published Online: Jun 24, 2021

Page range: 1 - 5

DOI: https://doi.org/10.21164/pomjlifesci.728

Keywordslow-level laser therapy, endothelial cell culture, t-PA, PAI-1, hyperglycemia

© 2021 Krzysztof Góralczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
low-level laser therapy, endothelial cell culture, t-PA, PAI-1, hyperglycemia